UniProt Accession

 PNG1_YEAST; Q02890; D6W3S1 

Genbank Protein ID

 U43281; BK006949 

Genbank Nucleotide ID

 AAB68203.1; DAA11337.1 

Protein Name

 Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase 

Protein Synonyms/Alias

 PNGase; Peptide:N-glycanase 1; yPNG1 

Gene Name

 PNG1 

Gene Synonyms/Alias

 YPL096W 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
33	FILSKFKKAAPVENI	ubiquitination	[1]
154	GPNGEESKFNCGTVE	ubiquitination	[1]
164	CGTVEIYKCNRCGNI	ubiquitination	[1]
285	ELPRDQIKEEDLKFL	ubiquitination	[1]
332	RGKTQETKSESVSAA	ubiquitination	[1]
341	ESVSAASKSSNRGRE	ubiquitination	[1]
355	ESGSADWKAQRGEDG	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. Involved in the formation of free oligosaccharide in cytosol. 

Sequence Annotation

 ACT_SITE 191 191 Nucleophile.
 ACT_SITE 218 218
 ACT_SITE 235 235
 METAL 129 129 Zinc.
 METAL 132 132 Zinc.
 METAL 165 165 Zinc.
 METAL 168 168 Zinc.
 BINDING 238 238 Substrate.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Hydrolase; Metal-binding; Nucleus; Reference proteome; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 363 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0003684; F:damaged DNA binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000224; F:peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity; IDA:SGD.
 GO:0006515; P:misfolded or incompletely synthesized protein catabolic process; IMP:SGD.
 GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
 GO:0006517; P:protein deglycosylation; IDA:SGD. 

Interpro

 IPR018325; Rad4/PNGase_transGLS-fold.
 IPR002931; Transglutaminase-like. 

Pfam

 PF03835; Rad4 

SMART

 SM00460; TGc 

PROSITE

  

PRINTS