CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018846
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-associated protein 3 
Protein Synonyms/Alias
 KAP-3; KAP3; Smg GDS-associated protein 
Gene Name
 KIFAP3 
Gene Synonyms/Alias
 KIF3AP; SMAP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
14RYLKRKVKGGNIDVHubiquitination[1]
59CQKIIRLKSLNANTDubiquitination[1, 2, 3, 4]
79RKVVEECKLIHPSKLubiquitination[1]
85CKLIHPSKLNEVEQLubiquitination[1]
246EELSKKKKAVDEDPEubiquitination[1]
469ICEGNGLKMLMKRALubiquitination[1]
477MLMKRALKFKDPLLMubiquitination[1, 3]
485FKDPLLMKMIRNISQubiquitination[1]
645LIDLMHDKNNEIRKVubiquitination[1]
651DKNNEIRKVCDNTLDubiquitination[1]
669EYDEEWAKKIQSEKFubiquitination[1]
670YDEEWAKKIQSEKFRubiquitination[1]
675AKKIQSEKFRWHNSQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex (By similarity). 
Sequence Annotation
 REPEAT 333 373 ARM 1.
 REPEAT 374 412 ARM 2.
 REPEAT 494 533 ARM 3.
 REPEAT 578 620 ARM 4.
 REPEAT 621 662 ARM 5.
 MOD_RES 60 60 Phosphoserine.  
Keyword
 Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 792 AA 
Protein Sequence
MQGEDARYLK RKVKGGNIDV HPSEKALIVH YEVEATILGE MGDPMLGERK ECQKIIRLKS 60
LNANTDITSL ARKVVEECKL IHPSKLNEVE QLLYYLQNRR DSLSGKEKKE KSSKPKDPPP 120
FEGMEIDEVA NINDMDEYIE LLYEDIPDKV RGSALILQLA RNPDNLEELL LNETALGALA 180
RVLREDWKQS VELATNIIYI FFCFSSFSQF HGLITHYKIG ALCMNIIDHE LKRHELWQEE 240
LSKKKKAVDE DPENQTLRKD YEKTFKKYQG LVVKQEQLLR VALYLLLNLA EDTRTELKMR 300
NKNIVHMLVK ALDRDNFELL ILVVSFLKKL SIFMENKNDM VEMDIVEKLV KMIPCEHEDL 360
LNITLRLLLN LSFDTGLRNK MVQVGLLPKL TALLGNDNYK QIAMCVLYHI SMDDRFKSMF 420
AYTDCIPQLM KMLFECSDER IDLELISFCI NLAANKRNVQ LICEGNGLKM LMKRALKFKD 480
PLLMKMIRNI SQHDGPTKNL FIDYVGDLAA QISNDEEEEF VIECLGTLAN LTIPDLDWEL 540
VLKEYKLVPY LKDKLKPGAA EDDLVLEVVI MIGTVSMDDS CAALLAKSGI IPALIELLNA 600
QQEDDEFVCQ IIYVFYQMVF HQATRDVIIK ETQAPAYLID LMHDKNNEIR KVCDNTLDII 660
AEYDEEWAKK IQSEKFRWHN SQWLEMVESR QMDESEQYLY GDDRIEPYIH EGDILERPDL 720
FYNSDGLIAS EGAISPDFFN DYHLQNGDVV GQHSFPGSLG MDGFGQPVGI LGRPATAYGF 780
RPDEPYYYGY GS 792 
Gene Ontology
 GO:0005813; C:centrosome; IDA:BHF-UCL.
 GO:0000794; C:condensed nuclear chromosome; IDA:BHF-UCL.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
 GO:0016939; C:kinesin II complex; ISS:UniProtKB.
 GO:0005876; C:spindle microtubule; IDA:BHF-UCL.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0072383; P:plus-end-directed vesicle transport along microtubule; TAS:BHF-UCL.
 GO:0046587; P:positive regulation of calcium-dependent cell-cell adhesion; IEA:Compara.
 GO:0006461; P:protein complex assembly; TAS:ProtInc.
 GO:0008104; P:protein localization; IEA:Compara.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo.
 IPR008658; KAP3. 
Pfam
  
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT 
PRINTS