CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017618
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Potassium voltage-gated channel subfamily H member 5 
Protein Synonyms/Alias
 Ether-a-go-go potassium channel 2; hEAG2; Voltage-gated potassium channel subunit Kv10.2 
Gene Name
 KCNH5 
Gene Synonyms/Alias
 EAG2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
712PVRKLFQKFKQQKELubiquitination[1]
785NRDAMELKPNGGADQubiquitination[2, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
 Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, Gong Y, Xu BE, Wu J, Luo Y, Qiang B, Yuan J, Sun X, Peng X.
 Proteomics. 2008 Jul;8(14):2885-96. [PMID: 18655026]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Pore-forming (alpha) subunit of voltage-gated potassium channel. Elicits a non-inactivating outward rectifying current. Channel properties may be modulated by cAMP and subunit assembly. 
Sequence Annotation
 DOMAIN 12 90 PAS.
 DOMAIN 91 143 PAC.
 NP_BIND 550 667 cNMP.
 REGION 704 715 Calmodulin-binding (Potential).
 REGION 909 948 CAD (involved in subunit assembly) (By
 MOTIF 432 437 Selectivity filter (By similarity).
 CARBOHYD 403 403 N-linked (GlcNAc...) (Potential).
 CROSSLNK 785 785 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Alternative splicing; Calmodulin-binding; Complete proteome; Glycoprotein; Ion channel; Ion transport; Isopeptide bond; Membrane; Polymorphism; Potassium; Potassium channel; Potassium transport; Reference proteome; Transmembrane; Transmembrane helix; Transport; Ubl conjugation; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 988 AA 
Protein Sequence
MPGGKRGLVA PQNTFLENIV RRSSESSFLL GNAQIVDWPV VYSNDGFCKL SGYHRADVMQ 60
KSSTCSFMYG ELTDKKTIEK VRQTFDNYES NCFEVLLYKK NRTPVWFYMQ IAPIRNEHEK 120
VVLFLCTFKD ITLFKQPIED DSTKGWTKFA RLTRALTNSR SVLQQLTPMN KTEVVHKHSR 180
LAEVLQLGSD ILPQYKQEAP KTPPHIILHY CAFKTTWDWV ILILTFYTAI MVPYNVSFKT 240
KQNNIAWLVL DSVVDVIFLV DIVLNFHTTF VGPGGEVISD PKLIRMNYLK TWFVIDLLSC 300
LPYDIINAFE NVDEGISSLF SSLKVVRLLR LGRVARKLDH YLEYGAAVLV LLVCVFGLVA 360
HWLACIWYSI GDYEVIDEVT NTIQIDSWLY QLALSIGTPY RYNTSAGIWE GGPSKDSLYV 420
SSLYFTMTSL TTIGFGNIAP TTDVEKMFSV AMMMVGSLLY ATIFGNVTTI FQQMYANTNR 480
YHEMLNNVRD FLKLYQVPKG LSERVMDYIV STWSMSKGID TEKVLSICPK DMRADICVHL 540
NRKVFNEHPA FRLASDGCLR ALAVEFQTIH CAPGDLIYHA GESVDALCFV VSGSLEVIQD 600
DEVVAILGKG DVFGDIFWKE TTLAHACANV RALTYCDLHI IKREALLKVL DFYTAFANSF 660
SRNLTLTCNL RKRIIFRKIS DVKKEEEERL RQKNEVTLSI PVDHPVRKLF QKFKQQKELR 720
NQGSTQGDPE RNQLQVESRS LQNGASITGT SVVTVSQITP IQTSLAYVKT SESLKQNNRD 780
AMELKPNGGA DQKCLKVNSP IRMKNGNGKG WLRLKNNMGA HEEKKEDWNN VTKAESMGLL 840
SEDPKSSDSE NSVTKNPLRK TDSCDSGITK SDLRLDKAGE ARSPLEHSPI QADAKHPFYP 900
IPEQALQTTL QEVKHELKED IQLLSCRMTA LEKQVAEILK ILSEKSVPQA SSPKSQMPLQ 960
VPPQIPCQDI FSVSRPESPE SDKDEIHF 988 
Gene Ontology
 GO:0009986; C:cell surface; IDA:MGI.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005622; C:intracellular; IDA:MGI.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
 GO:0005249; F:voltage-gated potassium channel activity; IEA:InterPro.
 GO:0010389; P:regulation of G2/M transition of mitotic cell cycle; IDA:MGI.
 GO:0023014; P:signal transduction by phosphorylation; IEA:GOC.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 
Interpro
 IPR018490; cNMP-bd-like.
 IPR000595; cNMP-bd_dom.
 IPR005821; Ion_trans_dom.
 IPR003949; K_chnl_volt-dep_EAG.
 IPR003938; K_chnl_volt-dep_EAG/ELK/ERG.
 IPR001610; PAC.
 IPR000014; PAS.
 IPR000700; PAS-assoc_C.
 IPR014710; RmlC-like_jellyroll. 
Pfam
 PF00027; cNMP_binding
 PF00520; Ion_trans 
SMART
 SM00100; cNMP
 SM00086; PAC
 SM00091; PAS 
PROSITE
 PS00888; CNMP_BINDING_1
 PS00889; CNMP_BINDING_2
 PS50042; CNMP_BINDING_3
 PS50113; PAC
 PS50112; PAS 
PRINTS
 PR01463; EAGCHANLFMLY.
 PR01464; EAGCHANNEL.