UniProt Accession

 SF3A1_MOUSE; Q8K4Z5; Q8C0M7; Q8C128; Q8C175; Q921T3 

Genbank Protein ID

 AK028829; AK029095; AK030223; AL807825; BC010727; BC029753 

Genbank Nucleotide ID

 BAC26142.1; BAC26294.1; BAC26853.1; CAI25745.1; AAH10727.1; AAH29753.1 

Protein Name

 Splicing factor 3A subunit 1 

Protein Synonyms/Alias

 SF3a120 

Gene Name

 Sf3a1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
55	EVRNIVDKTASFVAR	acetylation	[1, 2, 3]
80	QNEINNPKFNFLNPN	ubiquitination	[4]
188	FLTQLMQKEQRNYQF	acetylation	[1]
188	FLTQLMQKEQRNYQF	ubiquitination	[4]
223	TKILIPPKGLFSKLK	ubiquitination	[4]
251	CYRVEWAKFQERERK	acetylation	[1]
417	VSPITGEKIPASKMQ	ubiquitination	[4]
447	RDRSIREKQSDDEVY	ubiquitination	[4]
465	LDIESSLKQLAERRT	ubiquitination	[4]
484	VEETAIGKKIGEEEI	acetylation	[2]
529	EQIEAIHKAKGLVPE	ubiquitination	[4]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity). 

Sequence Annotation

 REPEAT 52 94 SURP motif 1.
 REPEAT 166 208 SURP motif 2.
 DOMAIN 705 788 Ubiquitin-like.
 MOD_RES 55 55 N6-acetyllysine (By similarity).
 MOD_RES 320 320 Phosphoserine (By similarity).
 MOD_RES 329 329 Phosphoserine.
 MOD_RES 357 357 Phosphoserine (By similarity).
 MOD_RES 411 411 Phosphoserine (By similarity).
 MOD_RES 449 449 Phosphoserine (By similarity).
 MOD_RES 454 454 Phosphotyrosine (By similarity).
 MOD_RES 757 757 Phosphotyrosine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Spliceosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 791 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005684; C:U2-type spliceosomal complex; ISS:UniProtKB.
 GO:0003723; F:RNA binding; ISS:UniProtKB.
 GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB. 

Interpro

 IPR022030; PRP21-like.
 IPR000061; Surp.
 IPR000626; Ubiquitin.
 IPR019955; Ubiquitin_supergroup. 

Pfam

 PF12230; PRP21_like_P
 PF01805; Surp
 PF00240; ubiquitin 

SMART

 SM00648; SWAP
 SM00213; UBQ 

PROSITE

 PS50128; SURP
 PS50053; UBIQUITIN_2 

PRINTS