UniProt Accession

 ENOB_MOUSE; P21550 

Genbank Protein ID

 X61600; X57747; X62667; AK002485; BC013460; M20745 

Genbank Nucleotide ID

 CAA43797.1; CAA40913.1; CAA44540.1; BAB22137.1; AAH13460.1; AAA37554.1 

Protein Name

 Beta-enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; MSE; Skeletal muscle enolase 

Gene Name

 Eno3 

Gene Synonyms/Alias

 Eno-3 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
5	***MAMQKIFAREIL	ubiquitination	[1]
28	EVDLHTAKGRFRAAV	ubiquitination	[1]
54	LELRDGDKARYLGKG	ubiquitination	[1]
60	DKARYLGKGVLKAVE	ubiquitination	[1]
64	YLGKGVLKAVEHINK	ubiquitination	[1]
71	KAVEHINKTLGPALL	ubiquitination	[1]
80	LGPALLEKKLSVVDQ	ubiquitination	[1]
81	GPALLEKKLSVVDQE	ubiquitination	[1]
92	VDQEKVDKFMIELDG	ubiquitination	[1]
179	PVGASSFKEAMRIGA	ubiquitination	[1]
256	SEFYRNGKYDLDFKS	ubiquitination	[1]
275	ARHISGEKLGELYKN	ubiquitination	[1]
420	IEEALGDKAVFAGRK	acetylation	[2]
420	IEEALGDKAVFAGRK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Appears to have a function in striated muscle development and regeneration. 

Sequence Annotation

 REGION 370 373 Substrate binding (By similarity).
 ACT_SITE 210 210 Proton donor (By similarity).
 ACT_SITE 343 343 Proton acceptor (By similarity).
 METAL 245 245 Magnesium (By similarity).
 METAL 293 293 Magnesium (By similarity).
 METAL 318 318 Magnesium (By similarity).
 BINDING 158 158 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 293 293 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).
 BINDING 394 394 Substrate (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Metal-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IEA:Compara.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IEA:EC.
 GO:0007568; P:aging; IEA:Compara.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0043403; P:skeletal muscle tissue regeneration; IEA:Compara. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.