CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030967
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division cycle protein 27 homolog 
Protein Synonyms/Alias
 cDNA FLJ57525, highly similar to Cell division cycle protein 27 homolog 
Gene Name
 CDC27 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38LSGGVFNKQKSHDDIubiquitination[1, 2, 3]
40GGVFNKQKSHDDIVTubiquitination[2, 3]
72CKTDRLAKGSECYQKubiquitination[2, 3]
269RIGQTGTKSVFSQSGubiquitination[2, 4]
329TSDSSTTKENSKKLKubiquitination[2]
354KTKSKTNKGGITQPNubiquitination[4]
370NDSLEITKLDSSIISubiquitination[4, 5]
380SSIISEGKISTITPQubiquitination[4]
496VALSVLSKDLTDMDKubiquitination[4]
503KDLTDMDKNSPEAWCubiquitination[2, 6]
527REHDIAIKFFQRAIQubiquitination[1, 2, 3, 6]
626HALKKSEKALDTLNKubiquitination[2, 3]
633KALDTLNKAIVIDPKubiquitination[1, 2, 3, 4]
640KAIVIDPKNPLCKFHubiquitination[2, 3]
645DPKNPLCKFHRASVLubiquitination[2]
657SVLFANEKYKSALQEubiquitination[2, 4]
659LFANEKYKSALQELEubiquitination[2, 3, 4]
669LQELEELKQIVPKESubiquitination[2, 4]
674ELKQIVPKESLVYFLubiquitination[1]
715KGANNQIKEAIDKRYubiquitination[2, 3]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094
Functional Description
  
Sequence Annotation
  
Keyword
 Cell cycle; Cell division; Complete proteome; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 763 AA 
Protein Sequence
MASTKPLCLP RCGFPRRTPL CRRLAEGEQI LSGGVFNKQK SHDDIVTEFG DSACFTLSLL 60
GHVYCKTDRL AKGSECYQKS LSLNPFLWSP FESLCEIGEK PDPDQTFKFT SLQNFSNCLP 120
NSCTTQVPNH SLSHRQPETV LTETPQDTIE LNRLNLESSN SKYSLNTDSS VSYIDSAVIS 180
PDTVPLGTGT SILSKQVQNK PKTGRSLLGG PAALSPLTPS FGILPLETPS PGDGSYLQNY 240
TNTPPVIDVP STGAPSKKSV ARIGQTGTKS VFSQSGNSRE VTPILAQTQS SGPQTSTTPQ 300
VLSPTITSPP NALPRRSSRL FTSDSSTTKE NSKKLKMKFP PKIPNRKTKS KTNKGGITQP 360
NINDSLEITK LDSSIISEGK ISTITPQIQA FNLQKAAAEG LMSLLREMGK GYLALCSYNC 420
KEAINILSHL PSHHYNTGWV LCQIGRAYFE LSEYMQAERI FSEVRRIENY RVEGMEIYST 480
TLWHLQKDVA LSVLSKDLTD MDKNSPEAWC AAGNCFSLQR EHDIAIKFFQ RAIQVDPNYA 540
YAYTLLGHEF VLTEELDKAL ACFRNAIRVN PRHYNAWYGL GMIYYKQEKF SLAEMHFQKA 600
LDINPQSSVL LCHIGVVQHA LKKSEKALDT LNKAIVIDPK NPLCKFHRAS VLFANEKYKS 660
ALQELEELKQ IVPKESLVYF LIGKVYKKLG QTHLALMNFS WAMDLDPKGA NNQIKEAIDK 720
RYLPDDEEPI TQEEQIMGTD ESQESSMTDA DDTQLHAAES DEF 763 
Gene Ontology
 GO:0005680; C:anaphase-promoting complex; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0031145; P:anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0051301; P:cell division; IEA:UniProtKB-KW. 
Interpro
 IPR026803; CDC27.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR013105; TPR_2.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1
 PF07719; TPR_2 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS