CPLM-011089

Genbank Nucleotide ID

Antigen peptide transporter 2

Protein Synonyms/Alias

APT2; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein

ABCB3; PSF2; RING11; Y1

Homo sapiens (Human)

Position	Peptide	Type	References
54	GGLWGLLKLRGLLGF	ubiquitination	[1]
135	EQDQVNNKVLMWRLL	ubiquitination	[1, 2]
245	LGFFQETKTGELNSR	ubiquitination	[1, 2, 3, 4, 5]
356	EHEVCRYKEALEQCR	ubiquitination	[1, 4]
603	IYTDVGEKGSQLAAG	ubiquitination	[1]
612	SQLAAGQKQRLAIAR	ubiquitination	[1]
679	ILVLQEGKLQKLAQL	ubiquitination	[1, 4]
682	LQEGKLQKLAQL***	ubiquitination	[4, 5, 6]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association.

DOMAIN 152 435 ABC transmembrane type-1.
DOMAIN 468 686 ABC transporter.
NP_BIND 503 510 ATP (Potential).
REGION 301 389 Involved in peptide-binding site.
REGION 414 433 Involved in peptide-binding site.

Adaptive immunity; Alternative splicing; ATP-binding; Complete proteome; Endoplasmic reticulum; Host-virus interaction; Immunity; Membrane; Nucleotide-binding; Peptide transport; Polymorphism; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; IDA:HPA.
GO:0005886; C:plasma membrane; IBA:RefGenome.
GO:0042825; C:TAP complex; IDA:UniProtKB.
GO:0005524; F:ATP binding; IDA:UniProtKB.
GO:0042288; F:MHC class I protein binding; IBA:RefGenome.
GO:0015421; F:oligopeptide-transporting ATPase activity; IBA:RefGenome.
GO:0042605; F:peptide antigen binding; IBA:RefGenome.
GO:0015433; F:peptide antigen-transporting ATPase activity; IMP:UniProtKB.
GO:0046978; F:TAP1 binding; IBA:RefGenome.
GO:0046979; F:TAP2 binding; IBA:RefGenome.
GO:0046980; F:tapasin binding; ISS:UniProtKB.
GO:0002485; P:antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; IBA:RefGenome.
GO:0002489; P:antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; IMP:UniProtKB.
GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
GO:0002481; P:antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; IBA:RefGenome.
GO:0046967; P:cytosol to ER transport; IMP:UniProtKB.
GO:0019060; P:intracellular transport of viral proteins in host cell; IMP:UniProtKB.
GO:0046968; P:peptide antigen transport; IDA:UniProtKB.
GO:0002591; P:positive regulation of antigen processing and presentation of peptide antigen via MHC class I; IBA:RefGenome.
GO:0001916; P:positive regulation of T cell mediated cytotoxicity; IBA:RefGenome.

IPR003593; AAA+_ATPase.
IPR013306; ABC_B3.
IPR003439; ABC_transporter-like.
IPR017871; ABC_transporter_CS.
IPR017940; ABC_transporter_type1.
IPR001140; ABC_transptr_TM_dom.
IPR011527; ABC_transptrTM_dom_typ1.
IPR005293; Ag_transporter2.
IPR027417; P-loop_NTPase.

PF00664; ABC_membrane
PF00005; ABC_tran

PS50929; ABC_TM1F
PS00211; ABC_TRANSPORTER_1
PS50893; ABC_TRANSPORTER_2

PR01897; TAP2PROTEIN.