UniProt Accession

 CDKAL_HUMAN; Q5VV42; A8K6S0; Q6P385; Q6ZR27; Q9NXB3 

Genbank Protein ID

 AK000349; AK128546; AK291735; AL513188; AL022717; AL033521; AL035090; AL451080; AL512405; AL513015; AL513549; AL451080; AL022717; AL033521; AL035090; AL512405; AL513015; AL513188; AL513549; AL512405; AL022717; AL033521; AL035090; AL451080; AL513015; AL513188; AL513549; AL513549; AL022717; AL033521; AL035090; AL451080; AL512405; AL513015; AL513188; AL022717; AL033521; AL035090; AL451080; AL512405; AL513015; AL513188; AL513549; AL035090; AL022717; AL033521; AL451080; AL512405; AL513015; AL513188; AL513549; AL033521; AL022717; AL035090; AL451080; AL512405; AL513015; AL513188; AL513549; AL513015; AL022717; AL033521; AL035090; AL451080; AL512405; AL513188; AL513549; CH471087; BC064145; BC121020; BC121021 

Genbank Nucleotide ID

 BAA91102.1; BAC87494.1; BAF84424.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72467.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH72474.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73074.1; CAH73706.1; CAH73706.1; CAH73706.1; CAH73706.1; CAH73706.1; CAH73706.1; CAH73706.1; CAH73706.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI19768.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI20280.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21023.1; CAI21687.1; CAI21687.1; CAI21687.1; CAI21687.1; CAI21687.1; CAI21687.1; CAI21687.1; CAI21687.1; EAW55426.1; AAH64145.1; AAI21021.1; AAI21022.1 

Protein Name

 Threonylcarbamoyladenosine tRNA methylthiotransferase 

Protein Synonyms/Alias

 CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase 

Gene Name

 CDKAL1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
45	VRRRNTQKYLQEEEN	ubiquitination	[1]
65	STIPGIQKIWIRTWG	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. 

Sequence Annotation

 DOMAIN 64 172 MTTase N-terminal.
 DOMAIN 431 493 TRAM.
 METAL 73 73 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 109 109 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 138 138 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 214 214 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 218 218 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 METAL 221 221 Iron-sulfur (4Fe-4S-S-AdoMet) (By
 MOD_RES 499 499 Phosphothreonine.  

Keyword

 4Fe-4S; Alternative splicing; Complete proteome; Diabetes mellitus; Endoplasmic reticulum; Iron; Iron-sulfur; Membrane; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; S-adenosyl-L-methionine; Transferase; Transmembrane; Transmembrane helix; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 579 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0009451; P:RNA modification; IEA:InterPro.
 GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. 

Interpro

 IPR006638; Elp3/MiaB/NifB.
 IPR023970; MeThioTfrase/rSAM.
 IPR005839; Methylthiotransferase.
 IPR020612; Methylthiotransferase_CS.
 IPR013848; Methylthiotransferase_N.
 IPR006466; MiaB-like_B.
 IPR007197; rSAM.
 IPR023404; rSAM_horseshoe.
 IPR002792; TRAM_dom. 

Pfam

 PF04055; Radical_SAM
 PF01938; TRAM
 PF00919; UPF0004 

SMART

 SM00729; Elp3 

PROSITE

 PS51449; MTTASE_N
 PS01278; MTTASE_RADICAL
 PS50926; TRAM 

PRINTS