UniProt Accession

 LDHA_RAT; P04642 

Genbank Protein ID

 X01964; BC084698; M54926 

Genbank Nucleotide ID

 CAA26000.1; AAH84698.1; AAA41508.1 

Protein Name

 L-lactate dehydrogenase A chain 

Protein Synonyms/Alias

 LDH-A; LDH muscle subunit; LDH-M 

Gene Name

 Ldha 

Gene Synonyms/Alias

 Ldh-1; Ldh1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
5	***MAALKDQLIVNL	acetylation	[1]
42	CAISILMKDLADELA	methylation	[2]
57	LVDVIEDKLKGEMMD	methylation	[2]
59	DVIEDKLKGEMMDLQ	methylation	[2]
76	SLFLKTPKIVSSKDY	ubiquitination	[3]
126	FIIPNVVKYSPQCKL	acetylation	[1]
155	WKISGFPKNRVIGSG	acetylation	[1]
224	QLGTDADKEQWKDVH	ubiquitination	[3]
232	EQWKDVHKQVVDSAY	acetylation	[1]
243	DSAYEVIKLKGYTSW	ubiquitination	[3]
278	HPISTMIKGLYGIKE	acetylation	[1]
317	PDEEARLKKSADTLW	acetylation	[1]
318	DEEARLKKSADTLWG	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561]
 [3] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

  

Sequence Annotation

 NP_BIND 29 57 NAD (By similarity).
 ACT_SITE 193 193 Proton acceptor (By similarity).
 BINDING 99 99 NAD (By similarity).
 BINDING 106 106 Substrate (By similarity).
 BINDING 138 138 NAD or substrate (By similarity).
 BINDING 169 169 Substrate (By similarity).
 BINDING 248 248 Substrate (By similarity).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 5 5 N6-acetyllysine (By similarity).
 MOD_RES 14 14 N6-acetyllysine (By similarity).
 MOD_RES 57 57 N6-acetyllysine (By similarity).
 MOD_RES 81 81 N6-acetyllysine (By similarity).
 MOD_RES 118 118 N6-acetyllysine (By similarity).
 MOD_RES 126 126 N6-acetyllysine (By similarity).
 MOD_RES 239 239 Phosphotyrosine (By similarity).
 MOD_RES 318 318 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 332 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0042802; F:identical protein binding; IDA:RGD.
 GO:0004459; F:L-lactate dehydrogenase activity; IDA:RGD.
 GO:0051287; F:NAD binding; IDA:RGD.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
 GO:0006089; P:lactate metabolic process; IDA:RGD.
 GO:0019674; P:NAD metabolic process; IDA:RGD.
 GO:0043065; P:positive regulation of apoptotic process; IDA:RGD.
 GO:0048569; P:post-embryonic organ development; IEP:RGD.
 GO:0051591; P:response to cAMP; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0043627; P:response to estrogen stimulus; IEP:RGD.
 GO:0009749; P:response to glucose stimulus; IEP:RGD.
 GO:0042542; P:response to hydrogen peroxide; IEP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD. 

Interpro

 IPR001557; L-lactate/malate_DH.
 IPR011304; L-lactate_DH.
 IPR018177; L-lactate_DH_AS.
 IPR022383; Lactate/malate_DH_C.
 IPR001236; Lactate/malate_DH_N.
 IPR015955; Lactate_DH/Glyco_Ohase_4_C.
 IPR016040; NAD(P)-bd_dom. 

Pfam

 PF02866; Ldh_1_C
 PF00056; Ldh_1_N 

SMART

  

PROSITE

 PS00064; L_LDH 

PRINTS

 PR00086; LLDHDRGNASE.