CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011632
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Bud site selection protein RAX1 
Protein Synonyms/Alias
 Revert to axial protein 1 
Gene Name
 RAX1 
Gene Synonyms/Alias
 YOR301W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
2******MKEELSKVSubiquitination[1]
140NELLGDWKHQSGIGQubiquitination[1]
172RRSQQDGKPQITTKQubiquitination[1]
274SVGVTNEKSNRSRGQubiquitination[1, 2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Required for the establishment of the bipolar budding pattern. Involved in selecting bud sites at both the distal and proximal poles of daughter cells as well as near previously used division sites on mother cells. Has a role in the localization of BUD8, the distal bipolar budding landmark, and of BUD9, the proximal pole landmark. 
Sequence Annotation
 DOMAIN 126 257 RGS.
 MOD_RES 167 167 Phosphoserine.  
Keyword
 Cell cycle; Cell division; Cell membrane; Complete proteome; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 435 AA 
Protein Sequence
MKEELSKVSS MQNFEMIQRE RLPTLYEVLI QRTSQPVDLW TFYTFLSQFP YAINYLDFWV 60
DLMTHTRLCK NYIELVRKSL INFPQEQQQN GSTSTATFDL LNALIEEGHL DPEAPDKLLE 120
NSGPDVPFSP KLNELLGDWK HQSGIGQEAL RNEDVALIVD EIMKRRSQQD GKPQITTKQL 180
LHSAVGLCNT YLVSPEQSER YLSNIPMETR NRIIESVQIE RKYDIEIFDD LKNLTYQFLE 240
MDCFPKFLSR VALHNIHDEI SDWRFHSVGV TNEKSNRSRG QTHISRSPFS NHTSISRIGF 300
GLLWLGIGFW IGYVLIFLAY SRAIRVVTVV PFTLGCYCIV CGMYQVDIVY SWFGVTQRLL 360
HRHKNAGNDE GDASSDTDHV PMILAVFGGR RRLTRIEHPF TRQLLRKRGL WCLLLVVGAT 420
AAFTVIFSCV PGRRV 435 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0000282; P:cellular bud site selection; IGI:SGD.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro. 
Interpro
 IPR000342; Regulat_G_prot_signal.
 IPR016137; Regulat_G_prot_signal_superfam. 
Pfam
  
SMART
 SM00315; RGS 
PROSITE
 PS50132; RGS 
PRINTS