CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007068
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein transport protein SEC31 
Protein Synonyms/Alias
 Protein WEB1 
Gene Name
 SEC31 
Gene Synonyms/Alias
 WEB1; YDL195W; D1229 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
428ALKTKDFKPLINQRLacetylation[1]
562VKNAYFAKYGSKSSLacetylation[1]
566YFAKYGSKSSLSRILubiquitination[2]
598SQWKFISKAIQNLYPubiquitination[2]
746RVLIASGKSLPAQNPubiquitination[2]
825APVHTEGKYAPPSQPacetylation[1]
1232HDLVALMKEKKYKEAubiquitination[2, 3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. 
Sequence Annotation
 REPEAT 6 46 WD 1.
 REPEAT 60 99 WD 2.
 REPEAT 106 146 WD 3.
 REPEAT 158 198 WD 4.
 REPEAT 207 250 WD 5.
 REPEAT 255 295 WD 6.
 REPEAT 298 338 WD 7.
 REPEAT 385 405 WD 8; interaction with SEC13.
 MOD_RES 349 349 Phosphoserine.
 MOD_RES 836 836 Phosphoserine.
 MOD_RES 974 974 Phosphoserine.
 MOD_RES 977 977 Phosphoserine.
 MOD_RES 980 980 Phosphoserine.
 MOD_RES 988 988 Phosphoserine.
 MOD_RES 992 992 Phosphoserine.
 MOD_RES 999 999 Phosphoserine.
 MOD_RES 1050 1050 Phosphothreonine.
 MOD_RES 1053 1053 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasmic vesicle; Endoplasmic reticulum; ER-Golgi transport; Membrane; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1273 AA 
Protein Sequence
MVKLAEFSRT ATFAWSHDKI PLLVSGTVSG TVDANFSTDS SLELWSLLAA DSEKPIASLQ 60
VDSKFNDLDW SHNNKIIAGA LDNGSLELYS TNEANNAINS MARFSNHSSS VKTVKFNAKQ 120
DNVLASGGNN GEIFIWDMNK CTESPSNYTP LTPGQSMSSV DEVISLAWNQ SLAHVFASAG 180
SSNFASIWDL KAKKEVIHLS YTSPNSGIKQ QLSVVEWHPK NSTRVATATG SDNDPSILIW 240
DLRNANTPLQ TLNQGHQKGI LSLDWCHQDE HLLLSSGRDN TVLLWNPESA EQLSQFPARG 300
NWCFKTKFAP EAPDLFACAS FDNKIEVQTL QNLTNTLDEQ ETETKQQESE TDFWNNVSRE 360
ESKEKPTVFH LQAPTWYGEP SPAAHWAFGG KLVQITPDGK GVSITNPKIS GLESNTTLSE 420
ALKTKDFKPL INQRLVKVID DVNEEDWNLL EKLSMDGTEE FLKEALAFDN DESDAQDDAN 480
NEKEDDGEEF FQQIETNFQP EGDFSLSGNI EQTISKNLVS GNIKSAVKNS LENDLLMEAM 540
VIALDSNNER LKESVKNAYF AKYGSKSSLS RILYSISKRE VDDLVENLDV SQWKFISKAI 600
QNLYPNDIAQ RNEMLIKLGD RLKENGHRQD SLTLYLAAGS LDKVASIWLS EFPDLEDKLK 660
KDNKTIYEAH SECLTEFIER FTVFSNFING SSTINNEQLI AKFLEFINLT TSTGNFELAT 720
EFLNSLPSDN EEVKTEKARV LIASGKSLPA QNPATATTSK AKYTNAKTNK NVPVLPTPGM 780
PSTTSIPSMQ APFYGMTPGA SANALPPKPY VPATTTSAPV HTEGKYAPPS QPSMASPFVN 840
KTNSSTRLNS FAPPPNPYAT ATVPATNVST TSIPQNTFAP IQPGMPIMGD YNAQSSSIPS 900
QPPINAVSGQ TPHLNRKAND GWNDLPLKVK EKPSRAKAVS VAPPNILSTP TPLNGIPANA 960
ASTMPPPPLS RAPSSVSMVS PPPLHKNSRV PSLVATSESP RASISNPYAP PQSSQQFPIG 1020
TISTANQTSN TAQVASSNPY APPPQQRVAT PLSGGVPPAP LPKASNPYAP TATTQPNGSS 1080
YPPTGPYTNN HTMTSPPPVF NKPPTGPPPI SMKKRSNKLA SIEQNPSQGA TYPPTLSSSA 1140
SPLQPSQPPT LASQVNTSAE NVSHEIPADQ QPIVDFLKEE LARVTPLTPK EYSKQLKDCD 1200
KRLKILFYHL EKQDLLTQPT IDCLHDLVAL MKEKKYKEAM VIHANIATNH AQEGGNWLTG 1260
VKRLIGIAEA TLN 1273 
Gene Ontology
 GO:0030127; C:COPII vesicle coat; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005198; F:structural molecule activity; IDA:SGD.
 GO:0090114; P:COPII-coated vesicle budding; IDA:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR021614; Sec31.
 IPR009917; SRA1-protein/COPII_Sec31.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF11549; Sec31
 PF07304; SRA1
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS