UniProt Accession

 NPR1_YEAST; P22211; D6W103 

Genbank Protein ID

 X56084; Z71459; BK006947 

Genbank Nucleotide ID

 CAA39564.1; CAA96076.1; DAA10369.1 

Protein Name

 Nitrogen permease reactivator protein 

Protein Synonyms/Alias

 Serine/threonine-protein kinase NPR1 

Gene Name

 NPR1 

Gene Synonyms/Alias

 YNL183C; N1631 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
44	LDLHSRNKSSSHIGS	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Nutrient-regulated protein kinase that promotes the activity of at least 6 distinct transport systems for nitrogenous nutrients under conditions of nitrogen catabolite derepression. Under poor nitrogen growth conditions, required for post-Golgi sorting of the general amino acid permease GAP1 and the three known ammonia permeases, MEP1/2/3, to the plasma membrane. Contributes also to the stability and the retention of GAP1 at the plasma membrane. Inversely, promotes the degradation of tryptophan permease TAT2 under the same conditions. Activity is regulated by the TOR signaling pathway via phosphatase SIT4. Although thought to be involved in regulation of GLN3-dependent transcription by nitrogen catabolite repression, this seems to be an indirect effect from the reduced uptake of the nitrogen-repressing compound. 

Sequence Annotation

 DOMAIN 438 742 Protein kinase.
 NP_BIND 444 452 ATP (By similarity).
 ACT_SITE 561 561 Proton acceptor (By similarity).
 BINDING 467 467 ATP (By similarity).
 MOD_RES 47 47 Phosphoserine; by autocatalysis.
 MOD_RES 85 85 Phosphoserine.
 MOD_RES 90 90 Phosphoserine.
 MOD_RES 100 100 Phosphoserine.
 MOD_RES 111 111 Phosphoserine.
 MOD_RES 116 116 Phosphoserine.
 MOD_RES 125 125 Phosphoserine.
 MOD_RES 137 137 Phosphoserine.
 MOD_RES 141 141 Phosphoserine.
 MOD_RES 257 257 Phosphoserine; by autocatalysis.
 MOD_RES 259 259 Phosphoserine.
 MOD_RES 260 260 Phosphoserine.
 MOD_RES 288 288 Phosphoserine.
 MOD_RES 292 292 Phosphoserine.
 MOD_RES 317 317 Phosphoserine.
 MOD_RES 320 320 Phosphoserine.
 MOD_RES 328 328 Phosphoserine.
 MOD_RES 334 334 Phosphotyrosine.
 MOD_RES 336 336 Phosphoserine.
 MOD_RES 353 353 Phosphoserine.
 MOD_RES 356 356 Phosphoserine.
 MOD_RES 357 357 Phosphoserine; by autocatalysis.
 MOD_RES 385 385 Phosphoserine.  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 790 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:SGD.
 GO:0005886; C:plasma membrane; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:SGD.
 GO:0045806; P:negative regulation of endocytosis; IMP:SGD.
 GO:0006808; P:regulation of nitrogen utilization; IMP:SGD.
 GO:0022898; P:regulation of transmembrane transporter activity; IMP:SGD. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS