UniProt Accession

 M0R629_RAT; M0R629 

Genbank Protein ID

 AABR06046382; CH474034 

Genbank Nucleotide ID

 EDL97426.1 

Protein Name

 Adenylosuccinate synthetase isozyme 1 

Protein Synonyms/Alias

 AMPSase 1; AdSS 1; Adenylosuccinate synthetase, basic isozyme; Adenylosuccinate synthetase, muscle isozyme; IMP--aspartate ligase 1 

Gene Name

 Adssl1 

Gene Synonyms/Alias

 Adss1; rCG_27771 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
18	PPGTGGVKRGRLQQE	acetylation	[1]
48	WGDEGKGKVVDLLAT	acetylation	[1]
115	GLFEEAEKNEKKGLK	acetylation	[1]
122	KNEKKGLKDWEKRLI	acetylation	[1]
158	QRQAQEGKNIGTTKK	acetylation	[1]
410	YETLPGWKADTTGAR	acetylation	[1]
448	VKWVGVGKSRESMIQ	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP (By similarity). 

Sequence Annotation

 NP_BIND 42 48 GTP (By similarity).
 NP_BIND 70 72 GTP (By similarity).
 NP_BIND 363 365 GTP (By similarity).
 NP_BIND 445 448 GTP (By similarity).
 REGION 43 46 IMP binding (By similarity).
 REGION 68 71 IMP binding (By similarity).
 REGION 331 337 Substrate binding (By similarity).
 ACT_SITE 43 43 Proton acceptor (By similarity).
 ACT_SITE 71 71 Proton donor (By similarity).
 METAL 43 43 Magnesium (By similarity).
 METAL 70 70 Magnesium; via carbonyl oxygen (By
 BINDING 43 43 Substrate (By similarity).
 BINDING 163 163 IMP (By similarity).
 BINDING 177 177 IMP; shared with dimeric partner (By
 BINDING 256 256 IMP (By similarity).
 BINDING 271 271 IMP (By similarity).
 BINDING 335 335 IMP (By similarity).
 BINDING 337 337 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 457 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0051015; F:actin filament binding; IDA:RGD.
 GO:0004019; F:adenylosuccinate synthase activity; IDA:RGD.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0003924; F:GTPase activity; IDA:RGD.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0006167; P:AMP biosynthetic process; IEA:InterPro.
 GO:0006531; P:aspartate metabolic process; IDA:RGD.
 GO:0035690; P:cellular response to drug; IEP:RGD.
 GO:0071257; P:cellular response to electrical stimulus; IEP:RGD.
 GO:0006541; P:glutamine metabolic process; IEP:RGD.
 GO:0046040; P:IMP metabolic process; IDA:RGD.
 GO:0009168; P:purine ribonucleoside monophosphate biosynthetic process; IDA:RGD.
 GO:0014850; P:response to muscle activity; IEP:RGD.
 GO:0042594; P:response to starvation; IEP:RGD. 

Interpro

 IPR018220; Adenylosuccinate_synthase_AS.
 IPR001114; Adenylosuccinate_synthetase.
 IPR027509; AdSS_1_vert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00709; Adenylsucc_synt 

SMART

 SM00788; Adenylsucc_synt 

PROSITE

 PS01266; ADENYLOSUCCIN_SYN_1
 PS00513; ADENYLOSUCCIN_SYN_2 

PRINTS