UniProt Accession

 RL2_ECOLI; P60422; P02387; Q2M6Y2 

Genbank Protein ID

 X02613; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA26463.1; AAA58114.1; AAC76342.1; BAE77974.1 

Protein Name

 50S ribosomal protein L2 

Protein Synonyms/Alias

  

Gene Name

 rplB 

Gene Synonyms/Alias

 b3317; JW3279 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
5	***MAVVKCKPTSPG	acetylation	[1]
18	PGRRHVVKVVNPELH	acetylation	[2]
26	VVNPELHKGKPFAPL	acetylation	[2]
28	NPELHKGKPFAPLLE	acetylation	[2]
36	PFAPLLEKNSKSGGR	acetylation	[2]
59	RHIGGGHKQAYRIVD	acetylation	[1]
97	NIALVLYKDGERRYI	acetylation	[2]
108	RRYILAPKGLKAGDQ	acetylation	[1, 2]
111	ILAPKGLKAGDQIQS	acetylation	[1, 2]
125	SGVDAAIKPGNTLPM	acetylation	[2]
147	TVHNVEMKPGKGGQL	acetylation	[2]
183	LRSGEMRKVEADCRA	acetylation	[1]
242	GEGRNFGKHPVTPWG	acetylation	[2, 3]
265	RSNKRTDKFIVRRRS	acetylation	[2]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 One of the primary rRNA binding proteins. Located near the base of the L1 stalk, it is probably also mobile. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is highly controversial. 

Sequence Annotation

 MOD_RES 242 242 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 273 AA 

Protein Sequence

Gene Ontology

 GO:0015934; C:large ribosomal subunit; IEA:InterPro.
 GO:0019843; F:rRNA binding; IEA:HAMAP.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0016740; F:transferase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IDA:EcoliWiki.
 GO:0006412; P:translation; IEA:HAMAP. 

Interpro

 IPR012340; NA-bd_OB-fold.
 IPR022666; Rbsml_prot_L2_RNA-bd_dom.
 IPR014722; Rib_L2_dom2.
 IPR002171; Ribosomal_L2.
 IPR005880; Ribosomal_L2_bac/org-type.
 IPR022669; Ribosomal_L2_C.
 IPR022671; Ribosomal_L2_CS.
 IPR014726; Ribosomal_L2_dom3.
 IPR008991; Translation_prot_SH3-like. 

Pfam

 PF00181; Ribosomal_L2
 PF03947; Ribosomal_L2_C 

SMART

  

PROSITE

 PS00467; RIBOSOMAL_L2 

PRINTS