CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017102
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exocyst complex component 8 
Protein Synonyms/Alias
 Exocyst complex 84 kDa subunit 
Gene Name
 EXOC8 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
29FEARLYVKQLSQQSDubiquitination[1, 2, 3]
60EETAQNLKRNVYQNYubiquitination[2, 4]
168TLTTLLEKVEGCRHLubiquitination[2]
268IFQAENAKIKREWLEubiquitination[2]
270QAENAKIKREWLEVLubiquitination[2]
281LEVLEDTKRALSEKRubiquitination[2]
426IRLGQCTKACELFLRubiquitination[2]
699RFEEGVGKPAKQLQDubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. 
Sequence Annotation
 DOMAIN 182 282 PH.
 MOD_RES 142 142 Phosphothreonine.  
Keyword
 Cell projection; Complete proteome; Cytoplasm; Exocytosis; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 725 AA 
Protein Sequence
MAMAMSDSGA SRLRRQLESG GFEARLYVKQ LSQQSDGDRD LQEHRQRIQA LAEETAQNLK 60
RNVYQNYRQF IETAREISYL ESEMYQLSHL LTEQKSSLES IPLTLLPAAA AAGAAAASGG 120
EEGVGGAGGR DHLRGQAGFF STPGGASRDG SGPGEEGKQR TLTTLLEKVE GCRHLLETPG 180
QYLVYNGDLV EYDADHMAQL QRVHGFLMND CLLVATWLPQ RRGMYRYNAL YSLDGLAVVN 240
VKDNPPMKDM FKLLMFPESR IFQAENAKIK REWLEVLEDT KRALSEKRRR EQEEAAAPRG 300
PPQVTSKATN PFEDDEEEEP AVPEVEEEKV DLSMEWIQEL PEDLDVCIAQ RDFEGAVDLL 360
DKLNHYLEDK PSPPPVKELR AKVEERVRQL TEVLVFELSP DRSLRGGPKA TRRAVSQLIR 420
LGQCTKACEL FLRNRAAAVH TAIRQLRIEG ATLLYIHKLC HVFFTSLLET AREFEIDFAG 480
TDSGCYSAFV VWARSAMGMF VDAFSKQVFD SKESLSTAAE CVKVAKEHCQ QLGDIGLDLT 540
FIIHALLVKD IQGALHSYKE IIIEATKHRN SEEMWRRMNL MTPEALGKLK EEMKSCGVSN 600
FEQYTGDDCW VNLSYTVVAF TKQTMGFLEE ALKLYFPELH MVLLESLVEI ILVAVQHVDY 660
SLRCEQDPEK KAFIRQNASF LYETVLPVVE KRFEEGVGKP AKQLQDLRNA SRLIRVNPES 720
TTSVV 725 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0000145; C:exocyst; IEA:Compara.
 GO:0030426; C:growth cone; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0016044; P:cellular membrane organization; TAS:Reactome.
 GO:0034613; P:cellular protein localization; IEA:Compara.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0006887; P:exocytosis; IEA:UniProtKB-KW.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR016159; Cullin_repeat-like_dom.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR014812; Vps51. 
Pfam
 PF08700; Vps51 
SMART
 SM00233; PH 
PROSITE
 PS50003; PH_DOMAIN 
PRINTS