CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015320
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 SPRY domain-containing SOCS box protein 3 
Protein Synonyms/Alias
 SSB-3 
Gene Name
 SPSB3 
Gene Synonyms/Alias
 C16orf31; SSB3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
142TAAIRGTKELGEGQHubiquitination[1, 2, 3]
325SMSCSRRKAPVSDPQubiquitination[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). 
Sequence Annotation
 DOMAIN 85 274 B30.2/SPRY.
 DOMAIN 264 315 SOCS box.  
Keyword
 Complete proteome; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MARRPRNSRA WHFVLSAARR DADARAVALA GSTNWGYDSD GQHSDSDSDP EYSTLPPSIP 60
SAVPVTGESF CDCAGQSEAS FCSSLHSAHR GRDCRCGEED EYFDWVWDDL NKSSATLLSC 120
DNRKVSFHME YSCGTAAIRG TKELGEGQHF WEIKMTSPVY GTDMMVGIGT SDVDLDKYRH 180
TFCSLLGRDE DSWGLSYTGL LHHKGDKTSF SSRFGQGSII GVHLDTWHGT LTFFKNRKCI 240
GVAATKLQNK RFYPMVCSTA ARSSMKVTRS CASATSLQYL CCHRLRQLRP DSGDTLEGLP 300
LPPGLKQVLH NKLGWVLSMS CSRRKAPVSD PQAATSAHPS SREPRPCQRK RCRRT 355 
Gene Ontology
 GO:0035556; P:intracellular signal transduction; IEA:InterPro.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR001496; SOCS_C.
 IPR003877; SPRY_rcpt. 
Pfam
 PF00622; SPRY 
SMART
 SM00969; SOCS_box 
PROSITE
 PS50188; B302_SPRY
 PS50225; SOCS 
PRINTS