CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-026480
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 CDK5 regulatory subunit associated protein 1 transcript variant 4 
Protein Synonyms/Alias
 CDK5 regulatory subunit-associated protein 1 
Gene Name
 CDK5RAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
70PTFQHFLKSASAPQEubiquitination[1, 2, 3]
78SASAPQEKLSSEVEDubiquitination[2, 3]
287ASILEEVKKLSEQGLubiquitination[2, 3]
288SILEEVKKLSEQGLKubiquitination[4]
295KLSEQGLKEVTLLGQubiquitination[2, 3, 4, 5, 6]
332RGFTTNYKTKQGGLRubiquitination[4]
364RFTSPHPKDFPDEVLubiquitination[1, 2, 5]
382HERDNICKQIHLPAQubiquitination[4, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 426 AA 
Protein Sequence
MHPLQCVLQV QRSLGWGPLA SVSWLSLRMC RAHSSLSSTM CPSPERQEDG ARKDFSSRLA 60
AGPTFQHFLK SASAPQEKLS SEVEDPPPYL MMDELLGRQR KVYLETYGCQ MNVNDTEIAW 120
SILQKSGYLR TSNLQEADVI LLVTCSIREK AEQTIWNRLH QLKALKTRRP RSRVPLRIGI 180
LGCMAERLKE EILNREKMVD ILAGPDAYRD LPRLLAVAES GQQAANVLLS LDETYADVMP 240
VQTSASATSA FVSIMRGCDN MCSYCIVPFT RGRERSRPIA SILEEVKKLS EQGLKEVTLL 300
GQNVNSFRDN SEVQFNSAVP TNLSRGFTTN YKTKQGGLRF AHLLDQVSRV DPEMRIRFTS 360
PHPKDFPDEV LQLIHERDNI CKQIHLPAQS GSSRVLEAMR RGYSREAYVE LVHHIRESIP 420
EDTGIS 426 
Gene Ontology
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
 GO:0016740; F:transferase activity; IEA:InterPro.
 GO:0009451; P:RNA modification; IEA:InterPro. 
Interpro
 IPR006638; Elp3/MiaB/NifB.
 IPR023970; MeThioTfrase/rSAM.
 IPR005839; Methylthiotransferase.
 IPR020612; Methylthiotransferase_CS.
 IPR013848; Methylthiotransferase_N.
 IPR007197; rSAM.
 IPR023404; rSAM_horseshoe. 
Pfam
 PF04055; Radical_SAM
 PF00919; UPF0004 
SMART
 SM00729; Elp3 
PROSITE
 PS51449; MTTASE_N
 PS01278; MTTASE_RADICAL 
PRINTS