CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004769
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aconitate hydratase, mitochondrial 
Protein Synonyms/Alias
 Aconitase; Citrate hydro-lyase 
Gene Name
 ACO1 
Gene Synonyms/Alias
 GLU1; YLR304C; L8003.22 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
27SNLTRDSKVNQNLLEacetylation[1]
144KRAIDLNKEVYDFLAacetylation[1]
229APKILGVKLTGKMNGacetylation[1]
254LAGITTVKGGTGKIVubiquitination[2]
316GKIADFAKLYHKDLLacetylation[1]
320DFAKLYHKDLLSADKacetylation[1]
398SRSASIVKDAAAHGLacetylation[1]
408AAHGLKSKTIFTVTPacetylation[1]
510RFNPLTDKLKDKDGNacetylation[1]
522DGNEFMLKPPHGDGLacetylation[1]
554DRSTVEVKVSPTSDRacetylation[1]
566SDRLQLLKPFKPWDGacetylation[1]
569LQLLKPFKPWDGKDAacetylation[1]
577PWDGKDAKDMPILIKacetylation[1]
584KDMPILIKAVGKTTTacetylation[1]
602SMAGPWLKYRGHLENacetylation[1]
624GAINAENKKANCVKNacetylation[1]
630NKKANCVKNVYTGEYacetylation[1]
638NVYTGEYKGVPDTARacetylation[1]
707GLLPLNFKNPADYDKacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Required for growth on nonfermentable carbon sources and for biosynthesis of glutamate. 
Sequence Annotation
 REGION 188 190 Substrate binding (By similarity).
 REGION 667 668 Substrate binding (By similarity).
 METAL 382 382 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 445 445 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 448 448 Iron-sulfur (4Fe-4S) (By similarity).
 BINDING 95 95 Substrate (By similarity).
 BINDING 471 471 Substrate (By similarity).
 BINDING 476 476 Substrate (By similarity).
 BINDING 604 604 Substrate (By similarity).
 MOD_RES 391 391 Phosphoserine.
 MOD_RES 409 409 Phosphothreonine.
 MOD_RES 556 556 Phosphoserine.  
Keyword
 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur; Lyase; Metal-binding; Mitochondrion; Phosphoprotein; Reference proteome; Transit peptide; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 778 AA 
Protein Sequence
MLSARSAIKR PIVRGLATVS NLTRDSKVNQ NLLEDHSFIN YKQNVETLDI VRKRLNRPFT 60
YAEKILYGHL DDPHGQDIQR GVSYLKLRPD RVACQDATAQ MAILQFMSAG LPQVAKPVTV 120
HCDHLIQAQV GGEKDLKRAI DLNKEVYDFL ASATAKYNMG FWKPGSGIIH QIVLENYAFP 180
GALIIGTDSH TPNAGGLGQL AIGVGGADAV DVMAGRPWEL KAPKILGVKL TGKMNGWTSP 240
KDIILKLAGI TTVKGGTGKI VEYFGDGVDT FSATGMGTIC NMGAEIGATT SVFPFNKSMI 300
EYLEATGRGK IADFAKLYHK DLLSADKDAE YDEVVEIDLN TLEPYINGPF TPDLATPVSK 360
MKEVAVANNW PLDVRVGLIG SCTNSSYEDM SRSASIVKDA AAHGLKSKTI FTVTPGSEQI 420
RATIERDGQL ETFKEFGGIV LANACGPCIG QWDRRDIKKG DKNTIVSSYN RNFTSRNDGN 480
PQTHAFVASP ELVTAFAIAG DLRFNPLTDK LKDKDGNEFM LKPPHGDGLP QRGYDAGENT 540
YQAPPADRST VEVKVSPTSD RLQLLKPFKP WDGKDAKDMP ILIKAVGKTT TDHISMAGPW 600
LKYRGHLENI SNNYMIGAIN AENKKANCVK NVYTGEYKGV PDTARDYRDQ GIKWVVIGDE 660
NFGEGSSREH AALEPRFLGG FAIITKSFAR IHETNLKKQG LLPLNFKNPA DYDKINPDDR 720
IDILGLAELA PGKPVTMRVH PKNGKPWDAV LTHTFNDEQI EWFKYGSALN KIKADEKK 778 
Gene Ontology
 GO:0005829; C:cytosol; IDA:SGD.
 GO:0005758; C:mitochondrial intermembrane space; TAS:Reactome.
 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0003994; F:aconitate hydratase activity; IMP:SGD.
 GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0003690; F:double-stranded DNA binding; IDA:SGD.
 GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003697; F:single-stranded DNA binding; IDA:SGD.
 GO:0000002; P:mitochondrial genome maintenance; IMP:SGD.
 GO:0006099; P:tricarboxylic acid cycle; IDA:SGD. 
Interpro
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS.
 IPR006248; Aconitase_mito-like.
 IPR000573; AconitaseA/IPMdHydase_ssu_swvl. 
Pfam
 PF00330; Aconitase
 PF00694; Aconitase_C 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.