UniProt Accession

 MUC18_HUMAN; P43121; O95812; Q59E86; Q6PHR3; Q6ZTR2 

Genbank Protein ID

 M29277; M28882; X68264; X68265; X68266; X68267; X68268; X68270; X68271; AF089868; AK126303; AB209925; BC056418 

Genbank Nucleotide ID

 AAA20824.1; AAA20922.1; CAA48332.1; CAA48332.1; CAA48332.1; CAA48332.1; CAA48332.1; CAA48332.1; CAA48332.1; AAD17799.1; BAC86520.1; BAD93162.1; AAH56418.1 

Protein Name

 Cell surface glycoprotein MUC18 

Protein Synonyms/Alias

 Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146 

Gene Name

 MCAM 

Gene Synonyms/Alias

 MUC18 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
595	LPCRRSGKQEITLPP	ubiquitination	[1]
613	SELVVEVKSDKLPEE	ubiquitination	[1, 2]
616	VVEVKSDKLPEEMGL	ubiquitination	[1, 2, 3, 4]
631	LQGSSGDKRAPGDQG	ubiquitination	[1, 2, 3]
640	APGDQGEKYIDLRH*	ubiquitination	[1, 3, 4]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 

Sequence Annotation

 DOMAIN 24 129 Ig-like V-type 1.
 DOMAIN 139 242 Ig-like V-type 2.
 DOMAIN 244 330 Ig-like C2-type 1.
 DOMAIN 335 424 Ig-like C2-type 2.
 DOMAIN 430 510 Ig-like C2-type 3.
 MOD_RES 614 614 Phosphoserine.
 MOD_RES 628 628 Phosphoserine.
 CARBOHYD 56 56 N-linked (GlcNAc...) (Potential).
 CARBOHYD 418 418 N-linked (GlcNAc...) (Potential).
 CARBOHYD 449 449 N-linked (GlcNAc...) (Potential).
 CARBOHYD 467 467 N-linked (GlcNAc...).
 CARBOHYD 508 508 N-linked (GlcNAc...) (Potential).
 CARBOHYD 518 518 N-linked (GlcNAc...) (Potential).
 CARBOHYD 527 527 N-linked (GlcNAc...) (Potential).
 CARBOHYD 544 544 N-linked (GlcNAc...) (Potential).
 DISULFID 48 116 Probable.
 DISULFID 161 223 Probable.
 DISULFID 272 320 Probable.
 DISULFID 365 407 Probable.
 DISULFID 452 499 Probable.  

Keyword

 Alternative splicing; Cell adhesion; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 646 AA 

Protein Sequence

Gene Ontology

 GO:0009897; C:external side of plasma membrane; IDA:UniProtKB.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0007155; P:cell adhesion; TAS:ProtInc.
 GO:0003094; P:glomerular filtration; IEP:UniProtKB.
 GO:0061042; P:vascular wound healing; IEP:UniProtKB. 

Interpro

 IPR013162; CD80_C2-set.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR003598; Ig_sub2.
 IPR013106; Ig_V-set.
 IPR013151; Immunoglobulin. 

Pfam

 PF08205; C2-set_2
 PF00047; ig
 PF07686; V-set 

SMART

 SM00409; IG
 SM00408; IGc2 

PROSITE

 PS50835; IG_LIKE 

PRINTS