UniProt Accession

 NFL_HUMAN; P07196; B9ZVN2; Q16154; Q8IU72 

Genbank Protein ID

 X05608; AY156690; AC107373; CH471080; CH471080; CH471080; BC039237; S70309 

Genbank Nucleotide ID

 CAA29097.1; AAN74826.1; EAW63598.1; EAW63599.1; EAW63600.1; AAH39237.1; AAD14057.1 

Protein Name

 Neurofilament light polypeptide 

Protein Synonyms/Alias

 NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein 

Gene Name

 NEFL 

Gene Synonyms/Alias

 NF68; NFL 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	PYYSTSYKRRYVETP	ubiquitination	[1, 2]
91	KSIRTQEKAQLQDLN	ubiquitination	[3]
157	AEDATNEKQALQGER	ubiquitination	[3]
271	QYEKLAAKNMQNAEE	ubiquitination	[1, 2]
370	SEMARYLKEYQDLLN	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REGION 2 92 Head.
 REGION 93 396 Rod.
 REGION 93 124 Coil 1A.
 REGION 125 137 Linker 1.
 REGION 138 234 Coil 1B.
 REGION 235 252 Linker 12.
 REGION 253 271 Coil 2A.
 REGION 272 280 Linker 2.
 REGION 281 396 Coil 2B.
 REGION 381 391 Epitope; recognized by IF-specific
 REGION 397 543 Tail.
 REGION 397 443 Tail, subdomain A.
 REGION 444 543 Tail, subdomain B (acidic).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 56 56 Phosphoserine (By similarity).
 MOD_RES 67 67 Phosphoserine (By similarity).
 MOD_RES 424 424 Phosphotyrosine (By similarity).
 MOD_RES 432 432 Phosphotyrosine (By similarity).
 MOD_RES 472 472 Phosphoserine (By similarity).
 CARBOHYD 21 21 O-linked (GlcNAc) (By similarity).
 CARBOHYD 27 27 O-linked (GlcNAc) (By similarity).  

Keyword

 Acetylation; Charcot-Marie-Tooth disease; Coiled coil; Complete proteome; Direct protein sequencing; Disease mutation; Glycoprotein; Intermediate filament; Neuropathy; Phosphoprotein; Polymorphism; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 543 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005883; C:neurofilament; IDA:UniProtKB.
 GO:0005200; F:structural constituent of cytoskeleton; IDA:UniProtKB.
 GO:0008089; P:anterograde axon cargo transport; IMP:UniProtKB.
 GO:0019896; P:axon transport of mitochondrion; IMP:UniProtKB.
 GO:0033693; P:neurofilament bundle assembly; IDA:UniProtKB.
 GO:0008090; P:retrograde axon cargo transport; IMP:UniProtKB.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS