CPLM-018746

Genbank Nucleotide ID

AP-3 complex subunit sigma-1

Protein Synonyms/Alias

AP-3 complex subunit sigma-3A; Adapter-related protein complex 3 sigma-1 subunit; Clathrin-associated/assembly/adapter protein, small 3; Sigma-3A-adaptin; Sigma3A-adaptin; Sigma-adaptin 3a

Homo sapiens (Human)

Position	Peptide	Type	References
13	LIFNNHGKPRLSKFY	ubiquitination	[1, 2, 3]
18	HGKPRLSKFYQPYSE	ubiquitination	[1, 2, 3, 4, 5]
41	ETFHLVSKRDENVCN	ubiquitination	[1]
149	DAQNKLEKSEAGLAG	ubiquitination	[1, 3]
166	ARAVSAVKNMNLPEI	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
185	NIGDISIKVPNLPSF	ubiquitination	[1, 2, 3, 7]

[1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.

Complete proteome; Cytoplasmic vesicle; Golgi apparatus; Membrane; Polymorphism; Protein transport; Reference proteome; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0030123; C:AP-3 adaptor complex; IDA:FlyBase.
GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
GO:0030133; C:transport vesicle; TAS:ProtInc.
GO:0008565; F:protein transporter activity; IEA:InterPro.
GO:0005215; F:transporter activity; NAS:ProtInc.
GO:0008089; P:anterograde axon cargo transport; ISS:UniProtKB.
GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
GO:0008286; P:insulin receptor signaling pathway; TAS:ProtInc.
GO:0006886; P:intracellular protein transport; IEA:InterPro.

IPR016635; AP_complex_ssu.
IPR022775; AP_mu_sigma_su.
IPR000804; Clathrin_sm-chain_CS.
IPR011012; Longin-like_dom.

PF01217; Clat_adaptor_s

PS00989; CLAT_ADAPTOR_S