CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002274
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hexokinase-1 
Protein Synonyms/Alias
 Brain form hexokinase; Hexokinase type I; HK I 
Gene Name
 Hk1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
62YNPTASVKMLPTFVRubiquitination[1]
187VEGADVVKLLNKAIKubiquitination[1]
191DVVKLLNKAIKKRGDubiquitination[1]
312LVRLILVKMAKEGLLacetylation[2]
333PELLTRGKFNTSDVSacetylation[2]
333PELLTRGKFNTSDVSubiquitination[1]
344SDVSAIEKDKEGIQNacetylation[2]
346VSAIEKDKEGIQNAKacetylation[2]
353KEGIQNAKEILTRLGacetylation[2]
418GVDGSLYKMHPQYSRacetylation[2]
488KQTLMEVKKRLRTEMubiquitination[1]
510TNSKATVKMLPSFVRubiquitination[1]
763NILIDFTKKGFLFRGubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
  
Sequence Annotation
 NP_BIND 84 89 ATP 1 (Potential).
 NP_BIND 425 426 ATP 1 (By similarity).
 NP_BIND 532 537 ATP 2 (By similarity).
 NP_BIND 747 748 ATP 2 (By similarity).
 NP_BIND 784 788 ATP 2 (By similarity).
 NP_BIND 863 867 ATP 2 (By similarity).
 REGION 1 12 Hydrophobic.
 REGION 13 475 Regulatory.
 REGION 84 88 Glucose-6-phosphate 1 binding.
 REGION 172 173 Substrate 1 binding.
 REGION 208 209 Substrate 1 binding.
 REGION 291 294 Substrate 1 binding.
 REGION 413 415 Glucose-6-phosphate 1 binding.
 REGION 476 918 Catalytic.
 REGION 532 536 Glucose-6-phosphate 2 binding.
 REGION 620 621 Substrate 2 binding.
 REGION 656 657 Substrate 2 binding.
 REGION 861 863 Glucose-6-phosphate 2 binding.
 BINDING 30 30 ATP 1 (By similarity).
 BINDING 155 155 Substrate 1.
 BINDING 209 209 Glucose-6-phosphate 1.
 BINDING 232 232 Glucose-6-phosphate 1.
 BINDING 235 235 Substrate 1.
 BINDING 260 260 Substrate 1.
 BINDING 449 449 Glucose-6-phosphate 1.
 BINDING 603 603 Glucose-6-phosphate 2.
 BINDING 657 657 Glucose-6-phosphate 2.
 BINDING 680 680 ATP 2 (By similarity).
 BINDING 680 680 Glucose-6-phosphate 2.
 BINDING 683 683 Substrate 2.
 BINDING 708 708 Substrate 2.
 BINDING 742 742 Substrate 2.
 BINDING 897 897 Glucose-6-phosphate 2.
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 27 27 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 918 AA 
Protein Sequence
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDEILIDIL TRFKKEMKNG LSRDYNPTAS 60
VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVSMESE IYDTPENIVH 120
GSGTQLFDHV ADCLGDFMEK KKIKDKKLPV GFTFSFPCRQ SKIDEAVLIT WTKRFKASGV 180
EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQQCEVGLII GTGTNACYME 240
ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE LDRGSLNPGK QLFEKMVSGM 300
YMGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKDKEGIQ NAKEILTRLG 360
VEPSDVDCVS VQHICTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKMH 420
PQYSRRFHKT LRRLVPDSDV RFLLSESGTG KGAAMVTAVA YRLAEQHRQI EETLAHFRLS 480
KQTLMEVKKR LRTEMEMGLR KETNSKATVK MLPSFVRSIP DGTEHGDFLA LDLGGTNFRV 540
LLVKIRSGKK RTVEMHNKIY SIPLEIMQGT GDELFDHIVS CISDFLDYMG IKGPRMPLGF 600
TFSFPCHQTN LDCGILISWT KGFKATDCEG HDVASLLRDA VKRREEFDLD VVAVVNDTVG 660
TMMTCAYEEP TCEIGLIVGT GTNACYMEEM KNVEMVEGNQ GQMCINMEWG AFGDNGCLDD 720
IRTDFDKVVD EYSLNSGKQR FEKMISGMYL GEIVRNILID FTKKGFLFRG QISEPLKTRG 780
IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSKRA AQLCGAGMAA 840
VVEKIRENRG LDHLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCTVSF LLSEDGSGKG 900
AALITAVGVR LRGDPSIA 918 
Gene Ontology
 GO:0005901; C:caveola; IDA:RGD.
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:RGD.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0005524; F:ATP binding; IDA:RGD.
 GO:0016887; F:ATPase activity; IC:RGD.
 GO:0004340; F:glucokinase activity; IDA:RGD.
 GO:0005536; F:glucose binding; IDA:RGD.
 GO:0032403; F:protein complex binding; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0004672; F:protein kinase activity; IDA:RGD.
 GO:0006096; P:glycolysis; IDA:RGD.
 GO:0043066; P:negative regulation of apoptotic process; IMP:RGD.
 GO:0018105; P:peptidyl-serine phosphorylation; IDA:RGD.
 GO:0018107; P:peptidyl-threonine phosphorylation; IDA:RGD.
 GO:0018108; P:peptidyl-tyrosine phosphorylation; IDA:RGD.
 GO:0046777; P:protein autophosphorylation; IDA:RGD.
 GO:0010359; P:regulation of anion channel activity; IDA:RGD. 
Interpro
 IPR001312; Hexokinase.
 IPR022673; Hexokinase_C.
 IPR019807; Hexokinase_CS.
 IPR022672; Hexokinase_N. 
Pfam
 PF00349; Hexokinase_1
 PF03727; Hexokinase_2 
SMART
  
PROSITE
 PS00378; HEXOKINASES 
PRINTS
 PR00475; HEXOKINASE.