CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018302
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dephospho-CoA kinase domain-containing protein 
Protein Synonyms/Alias
  
Gene Name
 DCAKD 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67ENGDINRKVLGDLIFubiquitination[1, 2, 3, 4, 5]
101EMMKETFKYFLRGYRubiquitination[1, 5]
121IPLLFETKKLLKYMKubiquitination[4, 6]
122PLLFETKKLLKYMKHubiquitination[4]
168AQLPLTDKARMARHVubiquitination[1, 4, 5, 6, 7]
186SGEWSVTKRQVILLHubiquitination[1, 4, 5, 6, 7]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
 DOMAIN 3 207 DPCK.
 NP_BIND 8 15 ATP (Potential).  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Nucleotide-binding; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 231 AA 
Protein Sequence
MFLVGLTGGI ASGKSSVIQV FQQLGCAVID VDVMARHVVQ PGYPAHRRIV EVFGTEVLLE 60
NGDINRKVLG DLIFNQPDRR QLLNAITHPE IRKEMMKETF KYFLRGYRYV ILDIPLLFET 120
KKLLKYMKHT VVVYCDRDTQ LARLMRRNSL NRKDAEARIN AQLPLTDKAR MARHVLDNSG 180
EWSVTKRQVI LLHTELERSL EYLPLRFGVL TGLAAIASLL YLLTHYLLPY A 231 
Gene Ontology
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004140; F:dephospho-CoA kinase activity; IEA:InterPro.
 GO:0015937; P:coenzyme A biosynthetic process; IEA:InterPro. 
Interpro
 IPR001977; Depp_CoAkinase.
 IPR027417; P-loop_NTPase. 
Pfam
 PF01121; CoaE 
SMART
  
PROSITE
 PS51219; DPCK 
PRINTS