| Tag | Content |
|---|
| CPLM ID | CPLM-004871 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | ATP-dependent RNA helicase DBP3 |
Protein Synonyms/Alias | DEAD box protein 3; Helicase CA3 |
Gene Name | DBP3 |
Gene Synonyms/Alias | YGL078C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 270 | EADRMLEKGFEEDIK | acetylation | [1] | | 350 | RKLLELLKKYHSGPK | acetylation | [1] | | 380 | ARVERNLKYNGYNVA | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs. |
Sequence Annotation | DOMAIN 143 315 Helicase ATP-binding.
DOMAIN 344 493 Helicase C-terminal.
NP_BIND 156 163 ATP (By similarity).
MOTIF 114 140 Q motif.
MOTIF 262 265 DEAD box. |
Keyword | ATP-binding; Complete proteome; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 523 AA |
Protein Sequence | MTKEEIADKK RKVVDEEVIE KKKSKKHKKD KKDKKEKKDK KHKKHKKEKK GEKEVEVPEK 60
ESEKKPEPTS AVASEFYVQS EALTSLPQSD IDEYFKENEI AVEDSLDLAL RPLLSFDYLS 120
LDSSIQAEIS KFPKPTPIQA VAWPYLLSGK DVVGVAETGS GKTFAFGVPA ISHLMNDQKK 180
RGIQVLVISP TRELASQIYD NLIVLTDKVG MQCCCVYGGV PKDEQRIQLK KSQVVVATPG 240
RLLDLLQEGS VDLSQVNYLV LDEADRMLEK GFEEDIKNII RETDASKRQT LMFTATWPKE 300
VRELASTFMN NPIKVSIGNT DQLTANKRIT QIVEVVDPRG KERKLLELLK KYHSGPKKNE 360
KVLIFALYKK EAARVERNLK YNGYNVAAIH GDLSQQQRTQ ALNEFKSGKS NLLLATDVAA 420
RGLDIPNVKT VINLTFPLTV EDYVHRIGRT GRAGQTGTAH TLFTEQEKHL AGGLVNVLNG 480
ANQPVPEDLI KFGTHTKKKE HSAYGSFFKD VDLTKKPKKI TFD 523 |
Gene Ontology | GO:0005730; C:nucleolus; IDA:SGD. GO:0030687; C:preribosome, large subunit precursor; IDA:SGD. GO:0005524; F:ATP binding; IEA:UniProtKB-KW. GO:0004004; F:ATP-dependent RNA helicase activity; IDA:SGD. GO:0003723; F:RNA binding; IEA:UniProtKB-KW. GO:0000464; P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. |
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