CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024473
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 RCC1 domain-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 RCCD1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
74SGAAGRCKDAWASEGubiquitination[1, 2]
271NEDGSQVKRTGGAEDubiquitination[1, 2, 3, 4, 5]
304PMGSDAVKASCGSRHubiquitination[2]
328LYTWGWGKYGQLGHEubiquitination[1, 2]
352RVEYFVDKQLQVKAVubiquitination[2]
373TYVYAVEKGKS****ubiquitination[2, 6, 7, 8]
375VYAVEKGKS******ubiquitination[2, 6, 8]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 REPEAT 6 56 RCC1 1.
 REPEAT 176 227 RCC1 2.
 REPEAT 229 317 RCC1 3.
 REPEAT 318 371 RCC1 4.  
Keyword
 Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 376 AA 
Protein Sequence
MAEERPGAWF GFGFCGFGQE LGSGRGRQVH SPSPLRAGVD ICRVSASWSY TAFVTRGGRL 60
ELSGSASGAA GRCKDAWASE GLLAVLRAGP GPEALLQVWA AESALRGEPL WAQNVVPEAE 120
GEDDPAGEAQ AGRLPLLPCA RAYVSPRAPF YRPLAPELRA RQLELGAEHA LLLDAAGQVF 180
SWGGGRHGQL GHGTLEAELE PRLLEALQGL VMAEVAAGGW HSVCVSETGD IYIWGWNESG 240
QLALPTRNLA EDGETVAREA TELNEDGSQV KRTGGAEDGA PAPFIAVQPF PALLDLPMGS 300
DAVKASCGSR HTAVVTRTGE LYTWGWGKYG QLGHEDTTSL DRPRRVEYFV DKQLQVKAVT 360
CGPWNTYVYA VEKGKS 376 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005886; C:plasma membrane; IDA:HPA. 
Interpro
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens. 
Pfam
 PF00415; RCC1 
SMART
  
PROSITE
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3 
PRINTS
 PR00633; RCCNDNSATION.