CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040523
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 DNA primase 
Protein Synonyms/Alias
  
Gene Name
 PRIM1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
71NNQSDLEKEMQKMNPubiquitination[1, 2]
80MQKMNPYKIDIGAVYubiquitination[2]
98PNQHNTVKLGAFQAQubiquitination[2, 3, 4, 5]
107GAFQAQEKELVFDIDubiquitination[1]
133SSADICPKCWTLMTMubiquitination[2]
150RIIDRALKEDFGFKHubiquitination[1, 2, 5]
156LKEDFGFKHRLWVYSubiquitination[2]
196VEYLSLVKGGQDVKKubiquitination[1, 2, 4, 5, 6]
210KKVHLSEKIHPFIRKubiquitination[2, 3]
217KIHPFIRKSINIIKKubiquitination[2]
240NQDILENKESWDKILubiquitination[1]
264ELQQSFQKSHNSLQRubiquitination[2, 3, 4]
314RLDINVSKGINHLLKubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; DNA replication; DNA-directed RNA polymerase; Primosome; Reference proteome; Transcription; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 328 AA 
Protein Sequence
METFDPTELP ELLKLYYRRL FPYSQYYRWL NYGGGDGVIK NYFQHREFSF TLKDDIYIRY 60
QSFNNQSDLE KEMQKMNPYK IDIGAVYSHR PNQHNTVKLG AFQAQEKELV FDIDMTDYDD 120
VRRCCSSADI CPKCWTLMTM AIRIIDRALK EDFGFKHRLW VYSGRRGVHC WVCDESVRKL 180
SSAVRSGIVE YLSLVKGGQD VKKKVHLSEK IHPFIRKSIN IIKKYFEEYA LVNQDILENK 240
ESWDKILALV PETIHDELQQ SFQKSHNSLQ RWEHLKKVAS RYQNNIKNDK YGPWLEWEIM 300
LQYCFPRLDI NVSKGINHLL KSPFSVHP 328 
Gene Ontology
 GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
 GO:0003896; F:DNA primase activity; IEA:InterPro. 
Interpro
 IPR002755; DNA_primase_S.
 IPR014052; DNA_primase_ssu_euk/arc. 
Pfam
 PF01896; DNA_primase_S 
SMART
  
PROSITE
  
PRINTS