UniProt Accession

 ACPH_HUMAN; P13798; Q9BQ33; Q9P0Y2 

Genbank Protein ID

 D38441; AF141383; BC000362; BC001499; BC001826; J03068 

Genbank Nucleotide ID

 BAA07476.1; AAF37321.1; AAH00362.1; AAH01499.1; AAH01826.1; AAA35769.1 

Protein Name

 Acylamino-acid-releasing enzyme 

Protein Synonyms/Alias

 AARE; Acyl-peptide hydrolase; APH; Acylaminoacyl-peptidase; Oxidized protein hydrolase; OPH 

Gene Name

 APEH 

Gene Synonyms/Alias

 D3F15S2; D3S48E; DNF15S2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
108	TMKAVLRKAGGTGPG	ubiquitination	[1]
118	GTGPGEEKQFLEVWE	ubiquitination	[1]
131	WEKNRKLKSFNLSAL	ubiquitination	[2]
180	AESFFQTKALDVSAS	ubiquitination	[1, 3, 4]
195	DDEIARLKKPDQAIK	ubiquitination	[1, 3]
664	IRYIPQVKTPLLLML	ubiquitination	[1, 3, 4]
681	EDRRVPFKQGMEYYR	ubiquitination	[1, 2, 4]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N- acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. 

Sequence Annotation

 ACT_SITE 587 587 Charge relay system.
 ACT_SITE 675 675 Charge relay system (By similarity).
 ACT_SITE 707 707 Charge relay system.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 185 185 Phosphoserine.
 MOD_RES 187 187 Phosphoserine.  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 732 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0031965; C:nuclear membrane; IDA:HPA.
 GO:0004252; F:serine-type endopeptidase activity; TAS:ProtInc.
 GO:0006508; P:proteolysis; IEA:InterPro. 

Interpro

 IPR011042; 6-blade_b-propeller_TolB-like.
 IPR002471; Pept_S9_AS.
 IPR001375; Peptidase_S9.
 IPR004106; Peptidase_S9A_B_C_N. 

Pfam

 PF00326; Peptidase_S9 

SMART

  

PROSITE

 PS00708; PRO_ENDOPEP_SER 

PRINTS