UniProt Accession

 MNMA_MYCPN; P75365 

Genbank Protein ID

 U00089 

Genbank Nucleotide ID

 AAB96067.1 

Protein Name

 tRNA-specific 2-thiouridylase MnmA 

Protein Synonyms/Alias

  

Gene Name

 mnmA 

Gene Synonyms/Alias

 trmU; MPN_422; MP419 

Created Date

 July 27, 2013 

Organism

 Mycoplasma pneumoniae (strain ATCC 29342 / M129) 

NCBI Taxa ID

 272634 

Lysine Modification

Position	Peptide	Type	References
117	MLCNRLIKFGLMRDY	acetylation	[1]

Reference

 [1] Cross-talk between phosphorylation and lysine acetylation in a genome-reduced bacterium.
 van Noort V, Seebacher J, Bader S, Mohammed S, Vonkova I, Betts MJ, Kühner S, Kumar R, Maier T, O'Flaherty M, Rybin V, Schmeisky A, Yus E, Stülke J, Serrano L, Russell RB, Heck AJ, Bork P, Gavin AC.
 Mol Syst Biol. 2012 Feb 28;8:571. [PMID: 22373819] 

Functional Description

 Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34 (By similarity). 

Sequence Annotation

 NP_BIND 9 16 ATP (By similarity).
 REGION 107 109 Interaction with target base in tRNA (By
 REGION 159 161 Interaction with tRNA (By similarity).
 ACT_SITE 112 112 Nucleophile (By similarity).
 ACT_SITE 209 209 Cysteine persulfide intermediate (By
 BINDING 35 35 ATP; via amide nitrogen and carbonyl
 BINDING 137 137 ATP; via amide nitrogen (By similarity).
 DISULFID 112 209 Alternate (By similarity).  

Keyword

 ATP-binding; Complete proteome; Cytoplasm; Disulfide bond; Nucleotide-binding; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 370 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016783; F:sulfurtransferase activity; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0006400; P:tRNA modification; IEA:HAMAP. 

Interpro

 IPR023382; Adenine_a_hdrlase_dom.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR004506; tRNA-specific_2-thiouridylase. 

Pfam

 PF03054; tRNA_Me_trans 

SMART

  

PROSITE

  

PRINTS