| Tag | Content |
|---|
| CPLM ID | CPLM-022724 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | E3 ubiquitin-protein ligase AMFR |
Protein Synonyms/Alias | Autocrine motility factor receptor; AMF receptor |
Gene Name | Amfr |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 353 | DSMQAARKLPCGHLF | ubiquitination | [1] | | 573 | ARGSRFSKSADERQR | ubiquitination | [1] | | 586 | QRMLVQRKDDLLQQA | ubiquitination | [1] | | 600 | ARKRFLNKSSEDDGA | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis (By similarity). |
Sequence Annotation | DOMAIN 456 498 CUE.
ZN_FING 341 379 RING-type.
REGION 614 643 VCP/p97-interacting motif (VIM).
MOD_RES 516 516 Phosphoserine (By similarity).
MOD_RES 542 542 Phosphoserine. |
Keyword | Alternative splicing; Complete proteome; Endoplasmic reticulum; Ligase; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Receptor; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 643 AA |
Protein Sequence | MPLLFLERFP WPSLRTYTGL SGLALLGTIV SAYRALSQPE DGSGEPEPLT APLQPEALAP 60
ARLTAGGPRA RDVAQYLLSD SLFVWVLVNT ACCVLMLVAK LIQCIVFGPL RVSERQHLKD 120
KFWNFIFYKF IFIFGVLNVQ TVEEVVMWCL WFAGLVFLHL MVQLCKDRFE YLSFSPTTPM 180
SSHGRVLSLL IAMLLSCCGL AVVCCVTGYT HGMHTLAFMA AESLLVTVRT AHVILRYVIH 240
LWDLNHEGTW EGKGTYVYYT DFVMELALLS LDLMHHIHML LFGNIWLSMA SLVIFMQLRY 300
LFHEVQRRIR RHKNYLRVVG NMEARFAVAT PEELAVNNDD CAICWDSMQA ARKLPCGHLF 360
HNSCLRSWLE QDTSCPTCRM SLNIADGSRA REDHQGENLD ENLVPVAAAE GRPRLNQHNH 420
FFHFDGSRIA SWLPSFSVEV MHTTNILGIT QASNSQLNAM AHQIQEMFPQ VPYHLVLQDL 480
QMTRSVEITT DNILEGRIQV PFPTQRSDSL RPALNSPVER PSPDLEEGEA SVQTERVPLD 540
LSPRLEETLD FSEVELEPIE VEDFEARGSR FSKSADERQR MLVQRKDDLL QQARKRFLNK 600
SSEDDGASER LLPSEGTSSD PVTLRRRMLA AAAERRLQRQ RTT 643 |
Gene Ontology | GO:0030176; C:integral to endoplasmic reticulum membrane; ISS:UniProtKB. GO:0005887; C:integral to plasma membrane; ISS:MGI. GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW. GO:0004872; F:receptor activity; IEA:Compara. GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:0030968; P:endoplasmic reticulum unfolded protein response; IEA:Compara. GO:0030433; P:ER-associated protein catabolic process; ISS:UniProtKB. GO:0051259; P:protein oligomerization; IEA:Compara. GO:0000209; P:protein polyubiquitination; ISS:UniProtKB. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |