CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001501
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HAUS augmin-like complex subunit 5 
Protein Synonyms/Alias
  
Gene Name
 HAUS5 
Gene Synonyms/Alias
 KIAA0841 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
77DSPQVRRKLELEAAVubiquitination[1, 2]
165QDMERKAKVDVTFGSubiquitination[1]
208NLLLPQAKRGSLPTPubiquitination[1, 2, 3, 4, 5, 6, 7]
313SQRSTLLKERQVLTQubiquitination[1, 4]
356CCLWTELKALHDQSQubiquitination[1]
382LLRELQAKQQRILHWubiquitination[4]
405EQVRLLIKGNSASKTubiquitination[1, 2, 4]
428VLALVQRKVVPTFEAubiquitination[1, 2, 6]
471RHRPGELKPLPTVLPubiquitination[1]
506KLGLPPGKASELLLPubiquitination[1, 2, 4, 5]
564ASQEKQQKENLGQALubiquitination[1]
572ENLGQALKRLEKLLKubiquitination[1]
576QALKRLEKLLKQALEubiquitination[1]
579KRLEKLLKQALERIPubiquitination[1]
630QAQGALQKLCS****ubiquitination[1, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. 
Sequence Annotation
  
Keyword
 Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 633 AA 
Protein Sequence
MELAQEAREL GCWAVEEMGV PVAARAPEST LRRLCLGQGA DIWAYILQHV HSQRTVKKIR 60
GNLLWYGHQD SPQVRRKLEL EAAVTRLRAE IQELDQSLEL MERDTEAQDT AMEQARQHTQ 120
DTQRRALLLR AQAGAMRRQQ HTLRDPMQRL QNQLRRLQDM ERKAKVDVTF GSLTSAALGL 180
EPVVLRDVRT ACTLRAQFLQ NLLLPQAKRG SLPTPHDDHF GTSYQQWLSS VETLLTNHPP 240
GHVLAALEHL AAEREAEIRS LCSGDGLGDT EISRPQAPDQ SDSSQTLPSM VHLIQEGWRT 300
VGVLVSQRST LLKERQVLTQ RLQGLVEEVE RRVLGSSERQ VLILGLRRCC LWTELKALHD 360
QSQELQDAAG HRQLLLRELQ AKQQRILHWR QLVEETQEQV RLLIKGNSAS KTRLCRSPGE 420
VLALVQRKVV PTFEAVAPQS RELLRCLEEE VRHLPHILLG TLLRHRPGEL KPLPTVLPSI 480
HQLHPASPRG SSFIALSHKL GLPPGKASEL LLPAAASLRQ DLLLLQDQRS LWCWDLLHMK 540
TSLPPGLPTQ ELLQIQASQE KQQKENLGQA LKRLEKLLKQ ALERIPELQG IVGDWWEQPG 600
QAALSEELCQ GLSLPQWRLR WVQAQGALQK LCS 633 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0070652; C:HAUS complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0051225; P:spindle assembly; IMP:UniProtKB. 
Interpro
 IPR026215; HAUS5. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR02091; HAUSAUGMINL5.