CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037267
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Nucleolar and spindle-associated protein 1 
Protein Synonyms/Alias
  
Gene Name
 NUSAP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
289VRFSAATKDNEHKRSacetylation[1]
327VLGTHKLKTITGNSAubiquitination[2]
372KQPHLQTKEEQRKKRacetylation[1, 3]
Reference
 [1] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 402 AA 
Protein Sequence
MIIPSLEELD SLKYSDLQNL AKSLGLRANL RATKLLKALK GYIKHEARKG NENQDESQTS 60
ASSCDETEIQ ISNQEEAERQ PLGHVTKTRR RCKTVRVDPD SQQNHSEIKI SNPTEFQNHE 120
KQESQDLRAT AKVPSPPDEH QEAENAVSSG NRDSKVPSEG KKSLYTDESS KPGKNKRTAI 180
TTPNFKKLHE AHFKEMESID QYIERKKKHF EEHNSMNELK QQPINKGGVR TPVPPRGRLS 240
VASTPISQRR SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT 300
PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAGKLKP WGQSKENNYL NQHVNRINFY 360
KKTYKQPHLQ TKEEQRKKRE QERKEKKAKV LGMRRGLILA ED 402 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005874; C:microtubule; IEA:InterPro.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005819; C:spindle; IEA:InterPro.
 GO:0040001; P:establishment of mitotic spindle localization; IEA:InterPro.
 GO:0000281; P:mitotic cytokinesis; IEA:InterPro. 
Interpro
 IPR026756; NuSAP. 
Pfam
  
SMART
  
PROSITE
  
PRINTS