CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000017
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Potassium channel subfamily K member 1 
Protein Synonyms/Alias
 Inward rectifying potassium channel protein TWIK-1; Potassium channel KCNO1 
Gene Name
 KCNK1 
Gene Synonyms/Alias
 HOHO1; KCNO1; TWIK1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
274FCELHELKKFRKMFYsumoylation[1, 2, 3]
316AGMKEDQKQNEPFVAubiquitination[4, 5, 6, 7]
Reference
 [1] Sumoylation silences the plasma membrane leak K+ channel K2P1.
 Rajan S, Plant LD, Rabin ML, Butler MH, Goldstein SA.
 Cell. 2005 Apr 8;121(1):37-47. [PMID: 15820677]
 [2] Potassium channel silencing by constitutive endocytosis and intracellular sequestration.
 Feliciangeli S, Tardy MP, Sandoz G, Chatelain FC, Warth R, Barhanin J, Bendahhou S, Lesage F.
 J Biol Chem. 2010 Feb 12;285(7):4798-805. [PMID: 19959478]
 [3] One SUMO is sufficient to silence the dimeric potassium channel K2P1.
 Plant LD, Dementieva IS, Kollewe A, Olikara S, Marks JD, Goldstein SA.
 Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10743-8. [PMID: 20498050]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Weakly inward rectifying potassium channel. 
Sequence Annotation
 MOD_RES 326 326 Phosphoserine (By similarity).
 CARBOHYD 95 95 N-linked (GlcNAc...) (Potential).
 DISULFID 69 69 Interchain.  
Keyword
 3D-structure; Complete proteome; Disulfide bond; Glycoprotein; Ion channel; Ion transport; Membrane; Phosphoprotein; Potassium; Potassium channel; Potassium transport; Reference proteome; Transmembrane; Transmembrane helix; Transport; Voltage-gated channel. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 336 AA 
Protein Sequence
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 60
RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 120
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRITVHV TRRPVLYFHI RWGFSKQVVA 180
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 240
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS 300
FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH 336 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0031526; C:brush border membrane; IEA:Compara.
 GO:0005768; C:endosome; IEA:Compara.
 GO:0008076; C:voltage-gated potassium channel complex; TAS:ProtInc.
 GO:0005242; F:inward rectifier potassium channel activity; TAS:ProtInc.
 GO:0035094; P:response to nicotine; IEA:Compara.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 
Interpro
 IPR003280; 2pore_dom_K_chnl.
 IPR022306; 2pore_dom_K_chnl_TASK/TWIK.
 IPR005408; 2pore_dom_K_chnl_TWIK.
 IPR001779; 2pore_dom_K_chnl_TWIK1.
 IPR013099; Ion_trans_2. 
Pfam
 PF07885; Ion_trans_2 
SMART
  
PROSITE
  
PRINTS
 PR01333; 2POREKCHANEL.
 PR01096; TWIK1CHANNEL.
 PR01586; TWIKCHANNEL.