CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030600
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate dehydrogenase 
Protein Synonyms/Alias
  
Gene Name
 GLUD1 
Gene Synonyms/Alias
 hCG_1993805 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16DVPFGGAKAGVKINPubiquitination[1]
20GGAKAGVKINPKNYTubiquitination[2]
24AGVKINPKNYTDNELubiquitination[1, 2, 3]
33YTDNELEKITRRFTMubiquitination[1, 2, 3]
44RFTMELAKKGFIGPGubiquitination[2, 3]
45FTMELAKKGFIGPGIubiquitination[1, 2, 3, 4]
159YLHRFGAKCIAVGESubiquitination[1]
179NPDGIDPKELEDFKLacetylation[5]
185PKELEDFKLQHGSILubiquitination[2, 3]
198ILGFPKAKPYEGSILubiquitination[1, 6]
232NAPRVKAKIIAEGANubiquitination[1, 2, 3]
248PTTPEADKIFLERNIacetylation[5]
248PTTPEADKIFLERNIubiquitination[1, 2, 3, 6]
290SYGRLTFKYERDSNYacetylation[4, 7]
290SYGRLTFKYERDSNYubiquitination[1]
313SLERKFGKHGGTIPIacetylation[4]
313SLERKFGKHGGTIPIubiquitination[2, 4]
336RISGASEKDIVHSGLacetylation[4, 5, 7]
336RISGASEKDIVHSGLubiquitination[1, 2, 3]
360QIMRTAMKYNLGLDLacetylation[4, 5, 7]
360QIMRTAMKYNLGLDLubiquitination[6]
378AYVNAIEKVFKVYNEubiquitination[1, 2, 3, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 391 AA 
Protein Sequence
MTYKCAVVDV PFGGAKAGVK INPKNYTDNE LEKITRRFTM ELAKKGFIGP GIDVPAPDMS 60
TGEREMSWIA DTYASTIGHY DINAHACVTG KPISQGGIHG RISATGRGVF HGIENFINEA 120
SYMSILGMTP GFGDKTFVVQ GFGNVGLHSM RYLHRFGAKC IAVGESDGSI WNPDGIDPKE 180
LEDFKLQHGS ILGFPKAKPY EGSILEADCD ILIPAASEKQ LTKSNAPRVK AKIIAEGANG 240
PTTPEADKIF LERNIMVIPD LYLNAGGVTV SYFEWLKNLN HVSYGRLTFK YERDSNYHLL 300
MSVQESLERK FGKHGGTIPI VPTAEFQDRI SGASEKDIVH SGLAYTMERS ARQIMRTAMK 360
YNLGLDLRTA AYVNAIEKVF KVYNEAGVTF T 391 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0016639; F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro. 
Interpro
 IPR006095; Glu/Leu/Phe/Val_DH.
 IPR006096; Glu/Leu/Phe/Val_DH_C.
 IPR006097; Glu/Leu/Phe/Val_DH_dimer_dom.
 IPR014362; Glu_DH.
 IPR016040; NAD(P)-bd_dom. 
Pfam
 PF00208; ELFV_dehydrog
 PF02812; ELFV_dehydrog_N 
SMART
 SM00839; ELFV_dehydrog 
PROSITE
 PS00074; GLFV_DEHYDROGENASE 
PRINTS
 PR00082; GLFDHDRGNASE.