UniProt Accession

 ZN363_HUMAN; Q96PM5; B3KRG3; C7E541; Q2KN33; Q59GN7; Q86X26; Q96PR5 

Genbank Protein ID

 GQ250944; AF247041; AF255666; AF305424; AB209072; AY888047; AK091501; AC096759; CH471057; BC047393 

Genbank Nucleotide ID

 ACT35531.1; AAL76101.1; AAK96896.1; AAL09356.1; BAD92309.1; AAX78233.1; BAG52375.1; EAX05725.1; AAH47393.1 

Protein Name

 RING finger and CHY zinc finger domain-containing protein 1 

Protein Synonyms/Alias

 Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; Zinc finger protein 363; p53-induced RING-H2 protein; hPirh2 

Gene Name

 RCHY1 

Gene Synonyms/Alias

 ARNIP; CHIMP; PIRH2; RNF199; ZNF363 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
30	YDRGCLLKAPCCDKL	ubiquitination	[1]
58	QLDRFKVKEVQCINC	ubiquitination	[1]
135	MNLQGRHKCIENVSR	ubiquitination	[1]
178	TCYEEMLKEGYRCPL	ubiquitination	[1]
241	HILGMKCKICESYNT	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, P73, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Monoubiquitinates the translesion DNA polymerase POLH. Contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. 

Sequence Annotation

 ZN_FING 13 80 CHY-type.
 ZN_FING 82 144 CTCHY-type.
 ZN_FING 145 189 RING-type.
 MOD_RES 257 257 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:HGNC.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0000151; C:ubiquitin ligase complex; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
 GO:0051865; P:protein autoubiquitination; IDA:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:UniProtKB. 

Interpro

 IPR004039; Rubredoxin-type_fold.
 IPR008913; Znf_CHY.
 IPR017921; Znf_CTCHY.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF05495; zf-CHY
 PF13639; zf-RING_2 

SMART

 SM00184; RING 

PROSITE

 PS51266; ZF_CHY
 PS51270; ZF_CTCHY
 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS