CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008923
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-soluble NSF attachment protein 
Protein Synonyms/Alias
 SNAP-alpha; N-ethylmaleimide-sensitive factor attachment protein alpha 
Gene Name
 NAPA 
Gene Synonyms/Alias
 SNAPA 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22AEAERKVKNSQSFFSubiquitination[1, 2, 3, 4]
93VDAGNAFKKADPQEAubiquitination[5]
153EQSADYYKGEESNSSubiquitination[1, 2, 3, 4, 5, 6, 7]
199AMDSPLLKYSAKDYFubiquitination[7]
203PLLKYSAKDYFFKAAacetylation[8]
203PLLKYSAKDYFFKAAubiquitination[1, 4]
227NAKLAVQKYEELFPAubiquitination[1, 2, 3, 4]
246RECKLMKKLLEAHEEubiquitination[2]
264DSYTESVKEYDSISRubiquitination[2, 9]
284TTMLLRIKKTIQGDEubiquitination[5]
285TMLLRIKKTIQGDEEubiquitination[2, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. 
Sequence Annotation
  
Keyword
 Complete proteome; ER-Golgi transport; Membrane; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 295 AA 
Protein Sequence
MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA 60
AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA 120
AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK 180
AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE 240
CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR 295 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0070044; C:synaptobrevin 2-SNAP-25-syntaxin-1a complex; IEA:Compara.
 GO:0045176; P:apical protein localization; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0006944; P:cellular membrane fusion; TAS:ProtInc.
 GO:0006891; P:intra-Golgi vesicle-mediated transport; TAS:ProtInc.
 GO:0006886; P:intracellular protein transport; IEA:Compara.
 GO:0030182; P:neuron differentiation; IEA:Compara.
 GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
 GO:0010807; P:regulation of synaptic vesicle priming; IEA:Compara.
 GO:0035494; P:SNARE complex disassembly; IEA:Compara.
 GO:0035249; P:synaptic transmission, glutamatergic; IEA:Compara. 
Interpro
 IPR000744; NSF_attach.
 IPR011990; TPR-like_helical. 
Pfam
  
SMART
  
PROSITE
  
PRINTS
 PR00448; NSFATTACHMNT.