CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001189
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial-processing peptidase subunit beta 
Protein Synonyms/Alias
 Beta-MPP; P-52 
Gene Name
 PMPCB 
Gene Synonyms/Alias
 MPPB 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
95GSRYENEKNNGTAHFubiquitination[1, 2]
144EQTVYYAKAFSKDLPubiquitination[3]
148YYAKAFSKDLPRAVEubiquitination[3, 4]
219LGPTENIKSISRKDLubiquitination[3, 4]
224NIKSISRKDLVDYITubiquitination[1, 2]
235DYITTHYKGPRIVLAubiquitination[1, 2, 3]
456TIREVCTKYIYNRSPubiquitination[4]
472IAAVGPIKQLPDFKQubiquitination[1, 2, 3, 4]
478IKQLPDFKQIRSNMCubiquitination[3, 4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Cleaves presequences (transit peptides) from mitochondrial protein precursors (By similarity). 
Sequence Annotation
 ACT_SITE 104 104 Proton acceptor (By similarity).
 METAL 101 101 Zinc (By similarity).
 METAL 105 105 Zinc (By similarity).
 METAL 181 181 Zinc (By similarity).  
Keyword
 Complete proteome; Direct protein sequencing; Hydrolase; Metal-binding; Metalloprotease; Mitochondrion; Polymorphism; Protease; Reference proteome; Transit peptide; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 489 AA 
Protein Sequence
MAAAAARVVL SSAARRRLWG FSESLLIRGA AGRSLYFGEN RLRSTQAATQ VVLNVPETRV 60
TCLESGLRVA SEDSGLSTCT VGLWIDAGSR YENEKNNGTA HFLEHMAFKG TKKRSQLDLE 120
LEIENMGAHL NAYTSREQTV YYAKAFSKDL PRAVEILADI IQNSTLGEAE IERERGVILR 180
EMQEVETNLQ EVVFDYLHAT AYQNTALGRT ILGPTENIKS ISRKDLVDYI TTHYKGPRIV 240
LAAAGGVSHD ELLDLAKFHF GDSLCTHKGE IPALPPCKFT GSEIRVRDDK MPLAHLAIAV 300
EAVGWAHPDT ICLMVANTLI GNWDRSFGGG MNLSSKLAQL TCHGNLCHSF QSFNTSYTDT 360
GLWGLYMVCE SSTVADMLHV VQKEWMRLCT SVTESEVARA RNLLKTNMLL QLDGSTPICE 420
DIGRQMLCYN RRIPIPELEA RIDAVNAETI REVCTKYIYN RSPAIAAVGP IKQLPDFKQI 480
RSNMCWLRD 489 
Gene Ontology
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0006626; P:protein targeting to mitochondrion; TAS:Reactome.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR011249; Metalloenz_LuxS/M16.
 IPR011237; Pept_M16_dom.
 IPR011765; Pept_M16_N.
 IPR001431; Pept_M16_Zn_BS.
 IPR007863; Peptidase_M16_C. 
Pfam
 PF00675; Peptidase_M16
 PF05193; Peptidase_M16_C 
SMART
  
PROSITE
 PS00143; INSULINASE 
PRINTS