CPLM-009255

Genbank Nucleotide ID

40S ribosomal protein S20

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
4	****MAFKDTGKTPV	ubiquitination	[1, 2, 3, 4]
8	MAFKDTGKTPVEPEV	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
30	TLTSRNVKSLEKVCA	ubiquitination	[2, 3, 6, 9]
34	RNVKSLEKVCADLIR	ubiquitination	[3]
49	GAKEKNLKVKGPVRM	ubiquitination	[1, 4]
51	KEKNLKVKGPVRMPT	ubiquitination	[1, 4]
59	GPVRMPTKTLRITTR	ubiquitination	[2]
67	TLRITTRKTPCGEGS	ubiquitination	[3]
75	TPCGEGSKTWDRFQM	ubiquitination	[3]

[1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[5] Quantitative analysis of global ubiquitination in HeLa cells by mass spectrometry.
Meierhofer D, Wang X, Huang L, Kaiser P.
J Proteome Res. 2008 Oct;7(10):4566-76. [PMID: 18781797]
[6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
[9] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]

Functional Description

MOD_RES 2 2 N-acetylalanine.
MOD_RES 9 9 Phosphothreonine.
CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)

3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0022627; C:cytosolic small ribosomal subunit; IDA:UniProtKB.
GO:0003723; F:RNA binding; NAS:UniProtKB.
GO:0003735; F:structural constituent of ribosome; IDA:UniProtKB.
GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; TAS:Reactome.
GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
GO:0006414; P:translational elongation; TAS:Reactome.
GO:0006413; P:translational initiation; TAS:Reactome.
GO:0006415; P:translational termination; TAS:Reactome.
GO:0019083; P:viral transcription; TAS:Reactome.

IPR001848; Ribosomal_S10.
IPR018268; Ribosomal_S10_CS.
IPR027486; Ribosomal_S10_dom.
IPR005729; Ribosomal_S10_euk/arc.

PF00338; Ribosomal_S10

PS00361; RIBOSOMAL_S10

PR00971; RIBOSOMALS10.