CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-037215
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit 
Protein Synonyms/Alias
  
Gene Name
 DDOST 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
145ADTENLLKAPTIVGKubiquitination[1, 2]
152KAPTIVGKSSLNPILubiquitination[1, 2, 3, 4, 5, 6]
264ALSRWVFKEEGVLRVubiquitination[1, 2, 3, 4, 5, 7]
354VYGVFQFKVDYNRLGubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 419 AA 
Protein Sequence
MGYFRCARAG SFGRRRKMEP STAARAWALF WLLLPLLGAV CASGPRTLVL LDNLNVRETH 60
SLFFRSLKDF GGNINVETIS AFIDGGGSVL VAASSDIGDP LRELGSECGI EFDEEKTAVI 120
DHHNYDISDL GQHTLIVADT ENLLKAPTIV GKSSLNPILF RGVGMVADPD NPLVLDILTG 180
SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF SGSLDFFSDS FFNSAVQKAA 240
PGSQRYSQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG ETAPPNAYTV TDLVEYSIVI 300
QQLSNGKWVP FDGDDIQLEF VRIDPFVRTF LKKKGGKYSV QFKLPDVYGV FQFKVDYNRL 360
GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFSMML GLFIFSIVFL HMKEKEKSD 419 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:InterPro.
 GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; IEA:InterPro.
 GO:0018279; P:protein N-linked glycosylation via asparagine; IEA:InterPro. 
Interpro
 IPR005013; WBP1. 
Pfam
 PF03345; DDOST_48kD 
SMART
  
PROSITE
  
PRINTS