CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024765
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ryanodine receptor 2 
Protein Synonyms/Alias
 RYR-2; RyR2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor 
Gene Name
 Ryr2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
304LRHVTTGKYLSLMEDubiquitination[1]
1253DITMWLSKRLPQFLQubiquitination[1]
1284IDSSPCLKVTQKSFGubiquitination[1]
1288PCLKVTQKSFGSQNNubiquitination[1]
1473TVTLGDEKGKVHESIubiquitination[1]
1562QFELGRIKNVMPLSAubiquitination[1]
1791PLDILKAKTIQMLTEubiquitination[1]
1801QMLTEAVKEGSLHARubiquitination[1]
1867EEGGTPEKEISIEDAubiquitination[1]
1875EISIEDAKLEGEEEAubiquitination[1]
2034RLLSLVEKVTYLKKKubiquitination[1]
2152LGDIMNNKVFYQHPNubiquitination[1]
2449PTIAKDGKVVEPDMSubiquitination[1]
2607EHAKMPLKLLTNHYEubiquitination[1]
2655FDALSQKKYEQELFKubiquitination[1]
2862KKLELESKGGGNHPLubiquitination[1]
2879PYDTLTAKEKAKDREubiquitination[1]
2907YVVSRGFKDLDLDTPubiquitination[1]
3075EKTMENLKQGQFTHTubiquitination[1]
3296IDEGAWMKRLAVFSQubiquitination[1]
3324HFLPLMEKLKKKAAMubiquitination[1]
3328LMEKLKKKAAMVVSEubiquitination[1]
3531RWQMALYKDLPNRTEubiquitination[1]
4384DIFGLDLKREGGQYKacetylation[2]
4391KREGGQYKLIPHNPNubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
 Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development. 
Sequence Annotation
 DOMAIN 110 165 MIR 1.
 DOMAIN 172 217 MIR 2.
 DOMAIN 225 280 MIR 3.
 DOMAIN 286 343 MIR 4.
 DOMAIN 351 408 MIR 5.
 DOMAIN 599 809 B30.2/SPRY 1.
 REPEAT 853 966 1.
 REPEAT 967 1080 2.
 DOMAIN 1025 1222 B30.2/SPRY 2.
 DOMAIN 1357 1563 B30.2/SPRY 3.
 REPEAT 2691 2809 3.
 REPEAT 2811 2924 4.
 REGION 853 2924 4 X approximate repeats.
 REGION 3580 3609 Interaction with CALM (By similarity).
 MOD_RES 2030 2030 Phosphoserine; by PKA.
 MOD_RES 2807 2807 Phosphoserine; by CaMK2D and PKA.
 MOD_RES 2813 2813 Phosphoserine; by CaMK2D.  
Keyword
 3D-structure; Calcium; Calcium channel; Calcium transport; Calmodulin-binding; Complete proteome; Developmental protein; Ion channel; Ion transport; Ligand-gated ion channel; Membrane; Phosphoprotein; Reference proteome; Repeat; Sarcoplasmic reticulum; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4966 AA 
Protein Sequence
MADAGEGEDE IQFLRTDDEV VLQCTATIHK EQQKLCLAAE GFGNRLCFLE STSNSKNVPP 60
DLSICTFVLE QSLSVRALQE MLANTVEKSE GQVDVEKWKF MMKTAQGGGH RTLLYGHAIL 120
LRHSYSGMYL CCLSTSRSST DKLAFDVGLQ EDTTGEACWW TIHPASKQRS EGEKVRVGDD 180
LILVSVSSER YLHLSYGNSS WHVDAAFQQT LWSVAPISSG SEAAQGYLIG GDVLRLLHGH 240
MDECLTVPSG EHGEEQRRTV HYEGGAVSVH ARSLWRLETL RVAWSGSHIR WGQPFRLRHV 300
TTGKYLSLME DKNLLLMDKE KADVKSTAFA FRSSKEKLDV GVRKEVDGMG TSEIKYGDSI 360
CYIQHVDTGL WLTYQAVDVK SARMGSIQRK AIMHHEGHMD DGLNLSRSQH EESRTARVIR 420
STVFLFNRFI RGLDALSKKV KLPTIDLPIE SVSLSLQDLI GYFHPPDEHL EHEDKQNRLR 480
ALKNRQNLFQ EEGMINLVLE CIDRLHVYSS AAHFADVAGR EAGESWKSIL NSLYELLAAL 540
IRGNRKNCAQ FSGSLDWLIS RLERLEASSG ILEVLHCVLV ESPEALNIIK EGHIKSIISL 600
LDKHGRNHKV LDVLCSLCVC HGVAVRSNQH LICDNLLPGR DLLLQTRLVN HVSSMRPNIF 660
LGVSEGSAQY KKWYYELMVD HTEPFVTAEA THLRVGWAST EGYSPYPGGG EEWGGNGVGD 720
DLFSYGFDGL HLWSGCIART VSSPNQHLLR TDDVISCCLD LSAPSISFRI NGQPVQGMFE 780
NFNIDGLFFP VVSFSAGIKV RFLLGGRHGE FKFLPPPGYA ACYEAVLPKE KLKVEHSREY 840
KQERTYTRDL LGPTVSLTQA AFTPVPVDTS QIVLPPHLER IRERLAENIH ELWVMNKIEL 900
GWQYGPVRDD NKRQHPCLVE FCKLPEQERN YNLQMSLETL KTLLALGCHV GIADEHAEEK 960
VKKMKLPKNY QLTSGYKPAP MDLSFIKLTP SQEAMVDKLA ENAHNVWARD RIRQGWTYGI 1020
QQDVKNRRNP RLVPYTLLDD RTKKSNKDSL REAVRTLLGY GYHLEAPDQD HASRAEVCSG 1080
TGERFRIFRA EKTYAVKAGR WYFEFEAVTA GDMRVGWSRP GCQPDLELGS DDRAFAFDGF 1140
KAQRWHQGNE HYGRSWQAGD VVGCMVDMNE HTMMFTLNGE ILLDDSGSEL AFKDFDVGDG 1200
FIPVCSLGVA QVGRMNFGKD VSTLKYFTIC GLQEGYEPFA VNTNRDITMW LSKRLPQFLQ 1260
VPSNHEHIEV TRIDGTIDSS PCLKVTQKSF GSQNNNTDIM FYRLSMPIEC AEVFSKSVAG 1320
GLPGAGFYGP KNDLEDFDVD SDFEVLMKTA HGHLVPDRID KDKETPKPEF NNHKDYAQEK 1380
PSRLKQRFLL RRTKPDYSTG HSARLTEDVL ADDRDDYEYL MQTSTYYYSV RIFPGQEPAN 1440
VWVGWITSDF HQYDTGFDLD RVRTVTVTLG DEKGKVHESI KRSNCYMVCA GESMSPGQGR 1500
NNSNGLEIGC VVDAASGLLT FIANGKELST YYQVEPSTKL FPAVFAQATS PNVFQFELGR 1560
IKNVMPLSAG LFKSEHKNPV PQCPPRLHVQ FLSHVLWSRM PNQFLKVDVS RISERQGWLV 1620
QCLDPLQFMS LHIPEENRSV DILELTEQEE LLQFHYHTLR LYSAVCALGN HRVAHALCSH 1680
VDEPQLLYAI ENKYMPGLLR AGYYDLLIDI HLSSYATARL MMNNEFIVPM TEETKSITLF 1740
PDENKKHGLP GIGLSTSLRP RMRFSSPSFV SISNDCYQYS PEFPLDILKA KTIQMLTEAV 1800
KEGSLHARDP VGGTTEFLFV PLIKLFYTLL IMGIFHNEDL KHILQLIEPS VFKEAAVPEE 1860
EGGTPEKEIS IEDAKLEGEE EAKGGKRPKE GLLQMKLPEP VKLQMCLLLQ YLCDCQVRHR 1920
IEAIVAFSDD FVAKLQDNQR FRYNEVMQAL NMSAALTARK TREFRSPPQE QINMLLNFKD 1980
DKSECPCPEE IRDQLLDFHE DLMTHCGIEL DEDGSLDGSN DLTIRGRLLS LVEKVTYLKK 2040
KQAEKPVASD SRKCSSLQQL ISETMVRWAQ ESVIEDPELV RAMFVLLHRQ YDGIGGLVRA 2100
LPKTYTINGV SVEDTINLLA SLGQIRSLLS VRMGKEEEKL MIRGLGDIMN NKVFYQHPNL 2160
MRALGMHETV MEVMVNVLGG GESKEITFPK MVANCCRFLC YFCRISRQNQ KAMFDHLSYL 2220
LENSSVGLAS PAMRGSTPLD VAAASVMDNN ELALALREPD LEKVVRYLAG CGLQSCQMLV 2280
SKGYPDIGWN PVEGERYLDF LRFAVFCNGE SVEENANVVV RLLIRRPECF GPALRGEGGN 2340
GLLAAMEEAI KIAEDPSRDG PSPTSGSSKT LDIEEEEDDT IHMGNAIMTF YAALIDLLGR 2400
CAPEMHLIHA GKGEAIRIRS ILRSLIPLGD LVGVISIAFQ MPTIAKDGKV VEPDMSAGFC 2460
PDHKAAMVLF LDRVYGIEVQ DFLLHLLEVG FLPDLRAAAS LDTAALSATD MALALNRYLC 2520
TAVLPLLTRC APLFAGTEHH ASLIDSLLHT VYRLSKGCSL TKAQRDSIEV CLLSICGQLR 2580
PSMMQHLLRR LVFDVPLLNE HAKMPLKLLT NHYERCWKYY CLPGGWGNFG AASEEELHLS 2640
RKLFWGIFDA LSQKKYEQEL FKLALPCLSA VAGALPPDYM ESNYVSMMEK QSSMDSEGNF 2700
NPQPVDTSNI TIPEKLEYFI NKYAEHSHDK WSMDKLANGW IYGEIYSDSS KIQPLMKPYK 2760
LLSEKEKEIY RWPIKESLKT MLAWGWRIER TREGDSMALY NRTRRISQTS QVSIDAAHGY 2820
SPRAIDMSNV TLSRDLHAMA EMMAENYHNI WAKKKKLELE SKGGGNHPLL VPYDTLTAKE 2880
KAKDREKAQD IFKFLQISGY VVSRGFKDLD LDTPSIEKRF AYSFLQQLIR YVDEAHQYIL 2940
EFDGGSRSKG EHFPYEQEIK FFAKVVLPLI DQYFKNHRLY FLSAASRPLC TGGHASNKEK 3000
EMVTSLFCKL GVLVRHRISL FGNDATSIVN CLHILGQTLD ARTVMKTGLD SVKSALRAFL 3060
DNAAEDLEKT MENLKQGQFT HTRSQPKGVT QIINYTTVAL LPMLSSLFEH IGQHQFGEDL 3120
ILEDVQVSCY RILTSLYALG TSKSIYVERQ RSALGECLAA FAGAFPIAFL ETHLDKHNVY 3180
SIYNTRSSRE RAALSLPANV EDVCPNIPSL EKLMTEIIEL AESGIRYTQM PYMMEVVLPM 3240
LCSYMSRWWE HGPENHPERA EMCCTALNSE HMNTLLGNIL KIIYNNLGID EGAWMKRLAV 3300
FSQPIINKVK PQLLKTHFLP LMEKLKKKAA MVVSEEDHLK AEARGDMSEA ELLILDEFTT 3360
LARDLYAFYP LLIRFVDYNR AKWLKEPNPE AEELFRMVAE VFIYWSKSHN FKREEQNFVV 3420
QNEINNMSFL ITDTKSKMSK AAISDQERKK MKRKGDRYSM QTSLIVAALK RLLPIGLNIC 3480
APGDQELIAL AKNRFSLKDT EEEVRDIIRS NIHLQGKLED PAIRWQMALY KDLPNRTEDP 3540
SDPERTVERV LGIANVLFHL EQKSKYTGRG YFSLVEHPQR SKKAVWHKLL SKQRKRAVVA 3600
CFRMAPLYNL PRHRAVNLFL QGYEKSWIET EEHYFEDKLI EDLAKPGAEL PEEDEAMKRV 3660
DPLHQLILLF SRTALTEKCK LEEDFLYMAY ADIMAKSCHD EEDDDGEEEV KSFEEKEMEK 3720
QKLLYQQARL HDRGAAEMVL QTISASKGET GPMVAATLKL GIAILNGGNS TVQQKMLDYL 3780
KEKKDVGFFQ SLAGLMQSCS VLDLNAFERQ NKAEGLGMVT EEGSGEKVLQ DDEFTCDLFR 3840
FLQLLCEGHN SDFQNYLRTQ TGNNTTVNII ISTVDYLLRV QESISDFYWY YSGKDIIDEQ 3900
GQRNFSKAIQ VAKQVFNTLT EYIQGPCTGN QQSLAHSRLW DAVVGFLHVF AHMQMKLSQD 3960
SSQIELLKEL MDLQKDMVVM LLSMLEGNVV NGTIGKQMVD MLVESSNNVE MILKFFDMFL 4020
KLKDLTSSDT FKEYDPDGKG VISKRDFHKA MESHKHYTQS ETEFLLSCAE TDENETLDYE 4080
EFVKRFHEPA KDIGFNVAVL LTNLSEHMPN DTRLQTFLEL AESVLNYFQP FLGRIEIMGS 4140
AKRIERVYFE ISESSRTQWE KPQVKESKRQ FIFDVVNEGG EKEKMELFVN FCEDTIFEMQ 4200
LAAQISESDL NERLANKEES EKERPEEQAP RMGFFSLLTI QSALFALRYN VLTLVRMLSL 4260
KSLKKQMKRM KKMTVKDMVL AFFSSYWSVF VTLLHFVASV CRGFFRIVSS LLLGGSLVEG 4320
AKKIKVAELL ANMPDPTQDE VRGDEEEGER KPLESALPSE DLTDLKELTE ESDLLSDIFG 4380
LDLKREGGQY KLIPHNPNAG LSDLMTNPVP VPEVQEKFQE QKAKEEKEEK EETKSEPEKA 4440
EGEDGEKEEK AKDEKSKQKL RQLHTHRYGE PEVPESAFWK KIIAYQQKLL NYFARNFYNM 4500
RMLALFVAFA INFILLFYKV STSSVVEGKE LPTRTSSDTA KVTNSLDSSP HRIIAVHYVL 4560
EESSGYMEPT LRILAILHTI ISFFCIIGYY CLKVPLVIFK REKEVARKLE FDGLYITEQP 4620
SEDDIKGQWD RLVINTQSFP NNYWDKFVKR KVMDKYGEFY GRDRISELLG MDKAALDFSD 4680
AREKKKPKKD SSLSAVLNSI DVKYQMWKLG VVFTDNSFLY LAWYMTMSVL GHYNNFFFAA 4740
HLLDIAMGFK TLRTILSSVT HNGKQLVLTV GLLAVVVYLY TVVAFNFFRK FYNKSEDGDT 4800
PDMKCDDMLT CYMFHMYVGV RAGGGIGDEI EDPAGDEYEI YRIIFDITFF FFVIVILLAI 4860
IQGLIIDAFG ELRDQQEQVK EDMETKCFIC GIGNDYFDTV PHGFETHTLQ EHNLANYLFF 4920
LMYLINKDET EHTGQESYVW KMYQERCWEF FPAGDCFRKQ YEDQLN 4966 
Gene Ontology
 GO:0034704; C:calcium channel complex; IEA:Compara.
 GO:0016021; C:integral to membrane; IC:MGI.
 GO:0043234; C:protein complex; ISO:MGI.
 GO:0033017; C:sarcoplasmic reticulum membrane; IDA:MGI.
 GO:0030018; C:Z disc; IDA:MGI.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005516; F:calmodulin binding; IDA:MGI.
 GO:0043621; F:protein self-association; IMP:UniProtKB.
 GO:0005219; F:ryanodine-sensitive calcium-release channel activity; IDA:MGI.
 GO:0043924; F:suramin binding; IEA:Compara.
 GO:0030509; P:BMP signaling pathway; IDA:MGI.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IDA:MGI.
 GO:0060048; P:cardiac muscle contraction; IEA:Compara.
 GO:0003300; P:cardiac muscle hypertrophy; IMP:MGI.
 GO:0071313; P:cellular response to caffeine; IDA:UniProtKB.
 GO:0005513; P:detection of calcium ion; IEA:Compara.
 GO:0003143; P:embryonic heart tube morphogenesis; IMP:UniProtKB.
 GO:0008627; P:intrinsic apoptotic signaling pathway in response to osmotic stress; IEA:Compara.
 GO:0003220; P:left ventricular cardiac muscle tissue morphogenesis; IMP:MGI.
 GO:0010460; P:positive regulation of heart rate; IMP:MGI.
 GO:0086094; P:positive regulation of ryanodine-sensitive calcium-release channel activity by adrenergic receptor signaling pathway involved in positive regulation of cardiac muscle contraction; IMP:BHF-UCL.
 GO:0086029; P:Purkinje myocyte to ventricular cardiac muscle cell signaling; IMP:BHF-UCL.
 GO:0010881; P:regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; IGI:BHF-UCL.
 GO:0086005; P:regulation of ventricular cardiac muscle cell action potential; IMP:BHF-UCL.
 GO:0014808; P:release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; IDA:BHF-UCL.
 GO:0001666; P:response to hypoxia; IDA:BHF-UCL.
 GO:0051775; P:response to redox state; IEA:Compara.
 GO:0097050; P:type B pancreatic cell apoptotic process; IMP:BHF-UCL. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR000699; Ca-rel_channel.
 IPR008985; ConA-like_lec_gl_sf.
 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom.
 IPR014821; Ins145_P3_rcpt.
 IPR005821; Ion_trans_dom.
 IPR016093; MIR_motif.
 IPR013662; RIH_assoc-dom.
 IPR013333; Ryan_recept.
 IPR003032; Ryanodine_rcpt.
 IPR015925; Ryanodine_recept-rel.
 IPR009460; Ryanrecept_TM4-6.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF08709; Ins145_P3_rec
 PF00520; Ion_trans
 PF02815; MIR
 PF08454; RIH_assoc
 PF06459; RR_TM4-6
 PF01365; RYDR_ITPR
 PF02026; RyR
 PF00622; SPRY 
SMART
 SM00054; EFh
 SM00472; MIR
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50919; MIR 
PRINTS
 PR00795; RYANODINER.