CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008528
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Kinesin-like protein KIF11 
Protein Synonyms/Alias
 Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5 
Gene Name
 KIF11 
Gene Synonyms/Alias
 EG5; KNSL1; TRIP5 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
34PFNLAERKASAHSIVubiquitination[1]
146TLHQIFEKLTDNGTEacetylation[2, 3]
207EEITVHNKDEVYQILubiquitination[1]
260EELVKIGKLNLVDLAubiquitination[1]
357LEYAHRAKNILNKPEubiquitination[1]
362RAKNILNKPEVNQKLubiquitination[1]
395DLAAAREKNGVYISEubiquitination[1]
508NTVEETTKDVSGLHSubiquitination[1]
516DVSGLHSKLDRKKAVubiquitination[4]
521HSKLDRKKAVDQHNAubiquitination[1]
550NNMEELIKDGSSKQKubiquitination[5]
672ADKIEDQKKELDGFLubiquitination[5]
712GNLTEDLKTIKQTHSubiquitination[1, 4]
715TEDLKTIKQTHSQELubiquitination[1, 4]
724THSQELCKLMNLWTEubiquitination[1]
745EKCENIQKPLSSVQEubiquitination[1]
757VQENIQQKSKDIVNKubiquitination[1]
771KMTFHSQKFCADSDGubiquitination[1]
792NFNQEGTKLVEESVKubiquitination[1, 4, 5]
803ESVKHSDKLNGNLEKubiquitination[1]
810KLNGNLEKISQETEQubiquitination[1, 5]
908TIKIGLTKLNCFLEQubiquitination[1, 6]
930TGTTPQRKSYLYPSTubiquitination[1]
952EHLLDQLKRKQPELLubiquitination[1, 4]
1034INTLERSKVEETTEHubiquitination[1, 4, 5, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. 
Sequence Annotation
 DOMAIN 16 363 Kinesin-motor.
 NP_BIND 105 112 ATP (By similarity).
 MOD_RES 146 146 N6-acetyllysine.
 MOD_RES 458 458 Phosphothreonine.
 MOD_RES 926 926 Phosphothreonine; by CDK1.
 MOD_RES 1033 1033 Phosphoserine; by NEK6.  
Keyword
 3D-structure; Acetylation; ATP-binding; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Microtubule; Mitosis; Motor protein; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1056 AA 
Protein Sequence
MASQPNSSAK KKEEKGKNIQ VVVRCRPFNL AERKASAHSI VECDPVRKEV SVRTGGLADK 60
SSRKTYTFDM VFGASTKQID VYRSVVCPIL DEVIMGYNCT IFAYGQTGTG KTFTMEGERS 120
PNEEYTWEED PLAGIIPRTL HQIFEKLTDN GTEFSVKVSL LEIYNEELFD LLNPSSDVSE 180
RLQMFDDPRN KRGVIIKGLE EITVHNKDEV YQILEKGAAK RTTAATLMNA YSSRSHSVFS 240
VTIHMKETTI DGEELVKIGK LNLVDLAGSE NIGRSGAVDK RAREAGNINQ SLLTLGRVIT 300
ALVERTPHVP YRESKLTRIL QDSLGGRTRT SIIATISPAS LNLEETLSTL EYAHRAKNIL 360
NKPEVNQKLT KKALIKEYTE EIERLKRDLA AAREKNGVYI SEENFRVMSG KLTVQEEQIV 420
ELIEKIGAVE EELNRVTELF MDNKNELDQC KSDLQNKTQE LETTQKHLQE TKLQLVKEEY 480
ITSALESTEE KLHDAASKLL NTVEETTKDV SGLHSKLDRK KAVDQHNAEA QDIFGKNLNS 540
LFNNMEELIK DGSSKQKAML EVHKTLFGNL LSSSVSALDT ITTVALGSLT SIPENVSTHV 600
SQIFNMILKE QSLAAESKTV LQELINVLKT DLLSSLEMIL SPTVVSILKI NSQLKHIFKT 660
SLTVADKIED QKKELDGFLS ILCNNLHELQ ENTICSLVES QKQCGNLTED LKTIKQTHSQ 720
ELCKLMNLWT ERFCALEEKC ENIQKPLSSV QENIQQKSKD IVNKMTFHSQ KFCADSDGFS 780
QELRNFNQEG TKLVEESVKH SDKLNGNLEK ISQETEQRCE SLNTRTVYFS EQWVSSLNER 840
EQELHNLLEV VSQCCEASSS DITEKSDGRK AAHEKQHNIF LDQMTIDEDK LIAQNLELNE 900
TIKIGLTKLN CFLEQDLKLD IPTGTTPQRK SYLYPSTLVR TEPREHLLDQ LKRKQPELLM 960
MLNCSENNKE ETIPDVDVEE AVLGQYTEEP LSQEPSVDAG VDCSSIGGVP FFQHKKSHGK 1020
DKENRGINTL ERSKVEETTE HLVTKSRLPL RAQINL 1056 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005871; C:kinesin complex; TAS:ProtInc.
 GO:0005874; C:microtubule; IDA:UniProtKB.
 GO:0005819; C:spindle; IDA:UniProtKB.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003777; F:microtubule motor activity; TAS:ProtInc.
 GO:0019886; P:antigen processing and presentation of exogenous peptide antigen via MHC class II; TAS:Reactome.
 GO:0007596; P:blood coagulation; TAS:Reactome.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007018; P:microtubule-based movement; TAS:Reactome.
 GO:0007100; P:mitotic centrosome separation; IEA:Compara.
 GO:0090307; P:spindle assembly involved in mitosis; IDA:UniProtKB. 
Interpro
 IPR025901; Kinesin-assoc_MT-bd_dom.
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin
 PF13931; Microtub_bind 
SMART
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.