UniProt Accession

 HIC1_HUMAN; Q14526; D3DTI4 

Genbank Protein ID

 L41919; AC090617; CH471108; CH471108 

Genbank Nucleotide ID

 AAD09201.1; EAW90562.1; EAW90563.1 

Protein Name

 Hypermethylated in cancer 1 protein 

Protein Synonyms/Alias

 Hic-1; Zinc finger and BTB domain-containing protein 29 

Gene Name

 HIC1 

Gene Synonyms/Alias

 ZBTB29 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
333	SLLYRWMKHEPGLGS	acetylation	[1]
333	SLLYRWMKHEPGLGS	sumoylation	[1, 2]

Reference

 [1] An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity.
 Stankovic-Valentin N, Deltour S, Seeler J, Pinte S, Vergoten G, Guérardel C, Dejean A, Leprince D.
 Mol Cell Biol. 2007 Apr;27(7):2661-75. [PMID: 17283066]
 [2] Differential regulation of HIC1 target genes by CtBP and NuRD, via an acetylation/SUMOylation switch, in quiescent versus proliferating cells.
 Van Rechem C, Boulay G, Pinte S, Stankovic-Valentin N, Guérardel C, Leprince D.
 Mol Cell Biol. 2010 Aug;30(16):4045-59. [PMID: 20547755] 

Functional Description

 Transcriptional repressor. Recognizes and binds to the consensus sequence '5-[CG]NG[CG]GGGCA[CA]CC-3'. May act as a tumor suppressor. May be involved in development of head, face, limbs and ventral body wall. Involved in down-regulation of SIRT1 and thereby is involved in regulation of p53/TP53-dependent apoptotic DNA-damage responses. The specific target gene promoter association seems to be depend on corepressors, such as CTBP1 or CTBP2 and MTA1. The regulation of SIRT1 transcription in response to nutrient deprivation seems to involve CTBP1. In cooperation with MTA1 (indicative for an association with the NuRD complex) represses transcription from CCND1/cyclin-D1 and CDKN1C/p57Kip2 specifically in quiescent cells. Involved in regulation of the Wnt signaling pathway probably by association with TCF7L2 and preventing TCF7L2 and CTNNB1 association with promoters of TCF- responsive genes. Seems to repress transcription from E2F1 and ATOH1 which involves ARID1A, indicative for the participation of a distinct SWI/SNF-type chromatin-remodeling complex. Probably represses transcription from CXCR7, FGFBP1 and EFNA1. 

Sequence Annotation

 DOMAIN 47 110 BTB.
 ZN_FING 439 459 C2H2-type 1.
 ZN_FING 509 529 C2H2-type 2.
 ZN_FING 537 557 C2H2-type 3.
 ZN_FING 565 585 C2H2-type 4.
 ZN_FING 593 613 C2H2-type 5.
 REGION 154 315 Mediates HDAC-dependent transcriptional
 REGION 241 247 Interaction with CTBP1.
 MOD_RES 313 313 Phosphoserine (By similarity).
 MOD_RES 333 333 N6-acetyllysine; alternate.
 CROSSLNK 333 333 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Developmental protein; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Tumor suppressor; Ubl conjugation; Wnt signaling pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 733 AA 

Protein Sequence

Gene Ontology

 GO:0000785; C:chromatin; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0008630; P:intrinsic apoptotic signaling pathway in response to DNA damage; IDA:UniProtKB.
 GO:0007275; P:multicellular organismal development; NAS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0030178; P:negative regulation of Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0043517; P:positive regulation of DNA damage response, signal transduction by p53 class mediator; ISS:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 

Interpro

 IPR000210; BTB/POZ-like.
 IPR011333; BTB/POZ_fold.
 IPR013069; BTB_POZ.
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 

Pfam

 PF00651; BTB
 PF00096; zf-C2H2 

SMART

 SM00225; BTB
 SM00355; ZnF_C2H2 

PROSITE

 PS50097; BTB
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 

PRINTS