UniProt Accession

 KC1D_HUMAN; P48730; A2I2P2; Q96KZ6; Q9BTN5 

Genbank Protein ID

 U29171; U31285; AB091044; AK291758; EF015900; BC003558; BC015775 

Genbank Nucleotide ID

 AAC50807.1; AAC50808.1; BAC10903.1; BAF84447.1; ABM64211.1; AAH03558.1; AAH15775.1 

Protein Name

 Casein kinase I isoform delta 

Protein Synonyms/Alias

 CKI-delta; CKId; Tau-protein kinase CSNK1D 

Gene Name

 CSNK1D 

Gene Synonyms/Alias

 HCKID 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
45	KLECVKTKHPQLHIE	ubiquitination	[1]
57	HIESKIYKMMQGGVG	ubiquitination	[1, 2, 3]
122	RIEYIHSKNFIHRDV	ubiquitination	[1, 2]
130	NFIHRDVKPDNFLMG	ubiquitination	[1, 2]
140	NFLMGLGKKGNLVYI	ubiquitination	[2]
141	FLMGLGKKGNLVYII	ubiquitination	[1]
155	IDFGLAKKYRDARTH	ubiquitination	[1]
171	HIPYRENKNLTGTAR	ubiquitination	[1]
232	YERISEKKMSTPIEV	ubiquitination	[1]
263	RSLRFDDKPDYSYLR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2, leading to retain PER1 in the cytoplasm. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA- binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate. 

Sequence Annotation

 DOMAIN 9 277 Protein kinase.
 NP_BIND 15 23 ATP (By similarity).
 REGION 278 364 Centrosomal localization signal (CLS).
 REGION 317 342 Autoinhibitory (By similarity).
 ACT_SITE 128 128 Proton acceptor (By similarity).
 BINDING 38 38 ATP (By similarity).
 MOD_RES 331 331 Phosphoserine.
 MOD_RES 370 370 Phosphoserine (By similarity).
 MOD_RES 382 382 Phosphoserine (By similarity).
 MOD_RES 383 383 Phosphoserine.
 MOD_RES 384 384 Phosphoserine.
 MOD_RES 411 411 Phosphoserine.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Biological rhythms; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Golgi apparatus; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Wnt signaling pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0005876; C:spindle microtubule; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0050321; F:tau-protein kinase activity; IEA:EC.
 GO:0032922; P:circadian regulation of gene expression; ISS:UniProtKB.
 GO:0006281; P:DNA repair; TAS:ProtInc.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0090263; P:positive regulation of canonical Wnt receptor signaling pathway; IMP:BHF-UCL.
 GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
 GO:0001934; P:positive regulation of protein phosphorylation; IMP:BHF-UCL.
 GO:0042752; P:regulation of circadian rhythm; ISS:UniProtKB.
 GO:0007165; P:signal transduction; TAS:ProtInc.
 GO:0051225; P:spindle assembly; IDA:UniProtKB.
 GO:0016055; P:Wnt receptor signaling pathway; IEA:UniProtKB-KW. 

Interpro

 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF00069; Pkinase 

SMART

  

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS