CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022502
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sentrin-specific protease 1 
Protein Synonyms/Alias
 Sentrin/SUMO-specific protease SENP1 
Gene Name
 SENP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
111SSSSSLQKSRNSRSLubiquitination[1]
124SLYLETRKTSSGLSNubiquitination[1]
153YEKSFPIKPVPSPSWubiquitination[1]
231SSSKNTLKDSLFKNGubiquitination[1, 2]
364LRQIEEQKALALQLQacetylation[3]
364LRQIEEQKALALQLQubiquitination[1]
396HLRVPLEKEIPVTVVacetylation[2, 3]
396HLRVPLEKEIPVTVVubiquitination[1]
408TVVQETQKKGHKLTDubiquitination[1]
577ESIDKKRKEFDTNGWubiquitination[4, 5]
590GWQLFSKKSQEIPQQubiquitination[1]
609DCGMFACKYADCITKubiquitination[1]
616KYADCITKDRPINFTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity. 
Sequence Annotation
 REGION 450 613 Protease.
 MOTIF 171 177 Nuclear localization signal (Probable).
 MOTIF 574 577 Nuclear localization signal (Potential).
 MOTIF 628 634 Nuclear localization signal (Potential).
 MOTIF 635 644 Nuclear export signal (Probable).
 ACT_SITE 533 533
 ACT_SITE 550 550
 ACT_SITE 603 603 Nucleophile.
 MOD_RES 157 157 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Cytoplasm; Hydrolase; Nucleus; Phosphoprotein; Polymorphism; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 644 AA 
Protein Sequence
MDDIADRMRM DAGEVTLVNH NSVFKTHLLP QTGFPEDQLS LSDQQILSSR QGHLDRSFTC 60
STRSAAYNPS YYSDNPSSDS FLGSGDLRTF GQSANGQWRN STPSSSSSLQ KSRNSRSLYL 120
ETRKTSSGLS NSFAGKSNHH CHVSAYEKSF PIKPVPSPSW SGSCRRSLLS PKKTQRRHVS 180
TAEETVQEEE REIYRQLLQM VTGKQFTIAK PTTHFPLHLS RCLSSSKNTL KDSLFKNGNS 240
CASQIIGSDT SSSGSASILT NQEQLSHSVY SLSSYTPDVA FGSKDSGTLH HPHHHHSVPH 300
QPDNLAASNT QSEGSDSVIL LKVKDSQTPT PSSTFFQAEL WIKELTSVYD SRARERLRQI 360
EEQKALALQL QNQRLQEREH SVHDSVELHL RVPLEKEIPV TVVQETQKKG HKLTDSEDEF 420
PEITEEMEKE IKNVFRNGNQ DEVLSEAFRL TITRKDIQTL NHLNWLNDEI INFYMNMLME 480
RSKEKGLPSV HAFNTFFFTK LKTAGYQAVK RWTKKVDVFS VDILLVPIHL GVHWCLAVVD 540
FRKKNITYYD SMGGINNEAC RILLQYLKQE SIDKKRKEFD TNGWQLFSKK SQEIPQQMNG 600
SDCGMFACKY ADCITKDRPI NFTQQHMPYF RKRMVWEILH RKLL 644 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0004175; F:endopeptidase activity; TAS:ProtInc.
 GO:0016929; F:SUMO-specific protease activity; ISS:UniProtKB.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IMP:UniProtKB.
 GO:0097190; P:apoptotic signaling pathway; IMP:UniProtKB.
 GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IGI:MGI.
 GO:0016926; P:protein desumoylation; ISS:UniProtKB.
 GO:0006508; P:proteolysis; TAS:ProtInc.
 GO:0010724; P:regulation of definitive erythrocyte differentiation; IEA:Compara. 
Interpro
 IPR003653; Peptidase_C48. 
Pfam
 PF02902; Peptidase_C48 
SMART
  
PROSITE
 PS50600; ULP_PROTEASE 
PRINTS