UniProt Accession

 SEC13_YEAST; Q04491; D6VYK9 

Genbank Protein ID

 L05929; U14913; BK006945 

Genbank Nucleotide ID

 AAA35028.1; AAB67426.1; DAA09525.1 

Protein Name

 Protein transport protein SEC13 

Protein Synonyms/Alias

  

Gene Name

 SEC13 

Gene Synonyms/Alias

 ANU3; YLR208W; L8167.4 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
172	NGTKESRKFVTGGAD	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Functions as a component of the nuclear pore complex (NPC) and the COPII coat. It is one of 5 proteins constituting the COPII coat, which is involved in anterograde (ER to Golgi) double- membrane transport vesicle formation. First the small GTPase SAR1, activated by and binding to the integral ER membrane protein SEC12, exchanges GDP for GTP and recruits the heterodimer SEC23/24, which in turn recruits the heterotetramer SEC13-SEC31. The polymerization of COPII coat complexes then causes physically the deformation (budding) of the membrane, leading to the creation of a transport vesicle. The COPII complex is dissociated upon SAR1-GTP hydrolysis to SAR1-GDP. SEC23 functions as the SAR1 GTPase activating protein, whose activity is stimulated in the presence of SEC13/31. SEC13 is directly or indirectly required for normal ER membrane and nuclear envelope morphology. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution. Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. 

Sequence Annotation

 REPEAT 7 46 WD 1.
 REPEAT 51 92 WD 2.
 REPEAT 97 138 WD 3.
 REPEAT 143 195 WD 4.
 REPEAT 202 244 WD 5.
 REPEAT 252 291 WD 6.  

Keyword

 3D-structure; Complete proteome; Cytoplasmic vesicle; Direct protein sequencing; Endoplasmic reticulum; ER-Golgi transport; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; Reference proteome; Repeat; Translocation; Transport; Vacuole; WD repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0030127; C:COPII vesicle coat; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0031080; C:nuclear pore outer ring; IDA:SGD.
 GO:0035859; C:Seh1-associated complex; IDA:SGD.
 GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
 GO:0005198; F:structural molecule activity; IDA:SGD.
 GO:0090114; P:COPII-coated vesicle budding; IMP:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
 GO:0031081; P:nuclear pore distribution; IMP:SGD.
 GO:0043547; P:positive regulation of GTPase activity; IDA:SGD.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:SGD.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 

Interpro

 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR017986; WD40_repeat_dom. 

Pfam

 PF00400; WD40 

SMART

 SM00320; WD40 

PROSITE

 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 

PRINTS