UniProt Accession

 TRML_ECOLI; P0AGJ7; P33899; Q2M7S0 

Genbank Protein ID

 L13970; U00039; U00096; AP009048; M34333 

Genbank Nucleotide ID

 AAB18583.1; AAC76630.1; BAE77686.1 

Protein Name

 tRNA (cytidine(34)-2'-O)-methyltransferase 

Protein Synonyms/Alias

 tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL 

Gene Name

 trmL 

Gene Synonyms/Alias

 yibK; b3606; JW3581 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
42	MGFAWDDKRLRRAGL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37. 

Sequence Annotation

 BINDING 78 78 S-adenosyl-L-methionine; via carbonyl
 BINDING 100 100 S-adenosyl-L-methionine; via amide
 BINDING 122 122 S-adenosyl-L-methionine; via amide
 BINDING 130 130 S-adenosyl-L-methionine (By similarity).  

Keyword

 Complete proteome; Cytoplasm; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 157 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0052666; F:tRNA (cytosine-2'-O-)-methyltransferase activity; IDA:EcoCyc.
 GO:0052665; F:tRNA (uracil-2'-O-)-methyltransferase activity; IDA:EcoCyc.
 GO:0002131; P:wobble position cytosine ribose methylation; IMP:EcoCyc.
 GO:0002132; P:wobble position uridine ribose methylation; IMP:EcoCyc. 

Interpro

 IPR001537; SpoU_MeTrfase.
 IPR016914; tRNA_cyt/urid_MeTfrase. 

Pfam

 PF00588; SpoU_methylase 

SMART

  

PROSITE

  

PRINTS