CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009244
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell division control protein 42 homolog 
Protein Synonyms/Alias
 G25K GTP-binding protein 
Gene Name
 Cdc42 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
5***MQTIKCVVVGDGacetylation[1]
96SFENVKEKWVPEITHubiquitination[2]
128DDPSTIEKLAKNKQKubiquitination[2]
133IEKLAKNKQKPITPEubiquitination[2]
135KLAKNKQKPITPETAacetylation[3]
135KLAKNKQKPITPETAubiquitination[2]
144ITPETAEKLARDLKAacetylation[3]
144ITPETAEKLARDLKAubiquitination[2]
153ARDLKAVKYVECSALacetylation[1]
153ARDLKAVKYVECSALubiquitination[2, 4]
163ECSALTQKGLKNVFDacetylation[5]
163ECSALTQKGLKNVFDsuccinylation[5]
163ECSALTQKGLKNVFDubiquitination[2]
183ALEPPEPKKSRRCVLubiquitination[2, 4]
184LEPPEPKKSRRCVLLubiquitination[2, 4]
Reference
 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [5] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration. 
Sequence Annotation
 NP_BIND 10 17 GTP (By similarity).
 NP_BIND 57 61 GTP (By similarity).
 NP_BIND 115 118 GTP (By similarity).
 MOTIF 32 40 Effector region (Potential).
 MOD_RES 64 64 Phosphotyrosine; by SRC (By similarity).
 MOD_RES 188 188 Cysteine methyl ester (By similarity).
 LIPID 188 188 S-geranylgeranyl cysteine (By  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Differentiation; Direct protein sequencing; GTP-binding; Lipoprotein; Membrane; Methylation; Neurogenesis; Nucleotide-binding; Phosphoprotein; Prenylation; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 191 AA 
Protein Sequence
MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG 60
QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR 120
DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP 180
EPKKSRRCVL L 191 
Gene Ontology
 GO:0045177; C:apical part of cell; IDA:MGI.
 GO:0071944; C:cell periphery; IDA:MGI.
 GO:0042995; C:cell projection; IDA:MGI.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030175; C:filopodium; IEA:Compara.
 GO:0000139; C:Golgi membrane; IEA:Compara.
 GO:0016020; C:membrane; ISS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0072686; C:mitotic spindle; ISS:UniProtKB.
 GO:0043005; C:neuron projection; IEA:Compara.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0030141; C:secretory granule; IEA:Compara.
 GO:0051233; C:spindle midzone; ISS:UniProtKB.
 GO:0005525; F:GTP binding; IDA:MGI.
 GO:0003924; F:GTPase activity; IDA:MGI.
 GO:0090135; P:actin filament branching; IEA:Compara.
 GO:0051017; P:actin filament bundle assembly; IMP:MGI.
 GO:0034332; P:adherens junction organization; IMP:MGI.
 GO:0060070; P:canonical Wnt receptor signaling pathway; IMP:MGI.
 GO:0003161; P:cardiac conduction system development; IGI:MGI.
 GO:0034613; P:cellular protein localization; IMP:MGI.
 GO:0016197; P:endosomal transport; TAS:UniProtKB.
 GO:0090136; P:epithelial cell-cell adhesion; IMP:MGI.
 GO:0060684; P:epithelial-mesenchymal cell signaling; IMP:MGI.
 GO:0051683; P:establishment of Golgi localization; IEA:Compara.
 GO:0035088; P:establishment or maintenance of apical/basal cell polarity; IMP:MGI.
 GO:0046847; P:filopodium assembly; IDA:MGI.
 GO:0007030; P:Golgi organization; IEA:Compara.
 GO:0031069; P:hair follicle morphogenesis; IMP:MGI.
 GO:0060789; P:hair follicle placode formation; IMP:MGI.
 GO:0060047; P:heart contraction; IGI:MGI.
 GO:0031424; P:keratinization; IMP:MGI.
 GO:0003334; P:keratinocyte development; IMP:MGI.
 GO:0035264; P:multicellular organism growth; IMP:MGI.
 GO:0010629; P:negative regulation of gene expression; IMP:MGI.
 GO:0031333; P:negative regulation of protein complex assembly; IEA:Compara.
 GO:0048664; P:neuron fate determination; IMP:MGI.
 GO:0007097; P:nuclear migration; IMP:MGI.
 GO:0072384; P:organelle transport along microtubule; IEA:Compara.
 GO:0032467; P:positive regulation of cytokinesis; ISS:UniProtKB.
 GO:0045740; P:positive regulation of DNA replication; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IMP:MGI.
 GO:0071338; P:positive regulation of hair follicle cell proliferation; IMP:MGI.
 GO:0090316; P:positive regulation of intracellular protein transport; IEA:Compara.
 GO:0046330; P:positive regulation of JNK cascade; IEA:Compara.
 GO:0043410; P:positive regulation of MAPK cascade; IDA:MGI.
 GO:0048554; P:positive regulation of metalloenzyme activity; IEA:Compara.
 GO:0043525; P:positive regulation of neuron apoptotic process; IEA:Compara.
 GO:0033138; P:positive regulation of peptidyl-serine phosphorylation; IMP:MGI.
 GO:0043552; P:positive regulation of phosphatidylinositol 3-kinase activity; IMP:MGI.
 GO:0031274; P:positive regulation of pseudopodium assembly; IEA:Compara.
 GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; ISS:UniProtKB.
 GO:0051835; P:positive regulation of synapse structural plasticity; IEA:Compara.
 GO:0051988; P:regulation of attachment of spindle microtubules to kinetochore; ISS:UniProtKB.
 GO:0051489; P:regulation of filopodium assembly; ISS:UniProtKB.
 GO:0007088; P:regulation of mitosis; IMP:MGI.
 GO:0042176; P:regulation of protein catabolic process; IMP:MGI.
 GO:0043497; P:regulation of protein heterodimerization activity; IDA:MGI.
 GO:0045859; P:regulation of protein kinase activity; IMP:MGI.
 GO:0031647; P:regulation of protein stability; IMP:MGI.
 GO:0007266; P:Rho protein signal transduction; TAS:MGI.
 GO:0002040; P:sprouting angiogenesis; IEA:Compara.
 GO:0060661; P:submandibular salivary gland formation; IEA:Compara. 
Interpro
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR001806; Small_GTPase.
 IPR003578; Small_GTPase_Rho. 
Pfam
 PF00071; Ras 
SMART
 SM00174; RHO 
PROSITE
 PS51420; RHO 
PRINTS
 PR00449; RASTRNSFRMNG.