CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008423
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 H(+)/Cl(-) exchange transporter 3 
Protein Synonyms/Alias
 Chloride channel protein 3; ClC-3; Chloride transporter ClC-3 
Gene Name
 CLCN3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
457LNTSELIKELFTDCGubiquitination[1]
698GFPVIMSKESQRLVGubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. 
Sequence Annotation
 DOMAIN 658 722 CBS 1.
 DOMAIN 755 812 CBS 2.
 NP_BIND 689 691 ATP (By similarity).
 NP_BIND 796 799 ATP (By similarity).
 MOTIF 238 242 Selectivity filter part_1 (By
 MOTIF 280 284 Selectivity filter part_2 (By
 MOTIF 525 529 Selectivity filter part_3 (By
 BINDING 239 239 Chloride (By similarity).
 BINDING 527 527 Chloride; via amide nitrogen (By
 BINDING 630 630 Chloride (By similarity).
 CARBOHYD 177 177 N-linked (GlcNAc...) (Potential).
 CARBOHYD 451 451 N-linked (GlcNAc...) (Potential).
 CARBOHYD 479 479 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Antiport; ATP-binding; CBS domain; Chloride; Complete proteome; Cytoplasmic vesicle; Endosome; Glycoprotein; Golgi apparatus; Ion transport; Membrane; Nucleotide-binding; Reference proteome; Repeat; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 818 AA 
Protein Sequence
MESEQLFHRG YYRNSYNSIT SASSDEELLD GAGVIMDFQT SEDDNLLDGD TAVGTHYTMT 60
NGGSINSSTH LLDLLDEPIP GVGTYDDFHT IDWVREKCKD RERHRRINSK KKESAWEMTK 120
SLYDAWSGWL VVTLTGLASG ALAGLIDIAA DWMTDLKEGI CLSALWYNHE QCCWGSNETT 180
FEERDKCPQW KTWAELIIGQ AEGPGSYIMN YIMYIFWALS FAFLAVSLVK VFAPYACGSG 240
IPEIKTILSG FIIRGYLGKW TLMIKTITLV LAVASGLSLG KEGPLVHVAC CCGNIFSYLF 300
PKYSTNEAKK REVLSAASAA GVSVAFGAPI GGVLFSLEEV SYYFPLKTLW RSFFAALVAA 360
FVLRSINPFG NSRLVLFYVE YHTPWYLFEL FPFILLGVFG GLWGAFFIRA NIAWCRRRKS 420
TKFGKYPVLE VIIVAAITAV IAFPNPYTRL NTSELIKELF TDCGPLESSS LCDYRNDMNA 480
SKIVDDIPDR PAGIGVYSAI WQLCLALIFK IIMTVFTFGI KVPSGLFIPS MAIGAIAGRI 540
VGIAVEQLAY YHHDWFIFKE WCEVGADCIT PGLYAMVGAA ACLGGVTRMT VSLVVIVFEL 600
TGGLEYIVPL MAAVMTSKWV GDAFGREGIY EAHIRLNGYP FLDAKEEFTH TTLAADVMRP 660
RRNDPPLAVL TQDNMTVDDI ENMINETSYN GFPVIMSKES QRLVGFALRR DLTIAIESAR 720
KKQEGIVGSS RVCFAQHTPS LPAESPRPLK LRSILDMSPF TVTDHTPMEI VVDIFRKLGL 780
RQCLVTHNGI VLGIITKKNI LEHLEQLKQH VEPLAPPWHY NKKRYPPAYG PDGKPRPRFN 840
NVQLNLTDEE REETEEEVYL LNSTTL 866 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0009986; C:cell surface; NAS:UniProtKB.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; TAS:UniProtKB.
 GO:0031902; C:late endosome membrane; TAS:Reactome.
 GO:0030658; C:transport vesicle membrane; IEA:UniProtKB-SubCell.
 GO:0012506; C:vesicle membrane; IDA:UniProtKB.
 GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0030165; F:PDZ domain binding; IDA:UniProtKB.
 GO:0046982; F:protein heterodimerization activity; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; NAS:UniProtKB.
 GO:0005247; F:voltage-gated chloride channel activity; TAS:ProtInc.
 GO:0048388; P:endosomal lumen acidification; TAS:UniProtKB. 
Interpro
 IPR014743; Cl-channel_core.
 IPR001807; Cl-channel_volt-gated.
 IPR002245; Cl_channel-3.
 IPR000644; Cysta_beta_synth_core. 
Pfam
 PF00571; CBS
 PF00654; Voltage_CLC 
SMART
 SM00116; CBS 
PROSITE
 PS51371; CBS 
PRINTS
 PR00762; CLCHANNEL.
 PR01114; CLCHANNEL3.