CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032127
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Aspartate aminotransferase 
Protein Synonyms/Alias
  
Gene Name
 GOT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
35WVLPVVKKVEQKIANubiquitination[1]
39VVKKVEQKIANDNSLubiquitination[2]
76GDDSPALKEKRVGGVubiquitination[1, 2]
78DSPALKEKRVGGVQSubiquitination[1]
109WYNGTNNKNTPVYVSubiquitination[2, 3]
133VFSAAGFKDIRSYRYubiquitination[2]
145YRYWDAEKRGLDLQGubiquitination[1, 4]
255GNLTVVGKEPESILQubiquitination[2]
269QVLSQMEKIVRITWSubiquitination[2]
304EEWTGNVKTMADRILubiquitination[1, 2, 3]
325RARLEALKTPGTWNHubiquitination[2]
357VEYLVNEKHIYLLPSacetylation[5]
357VEYLVNEKHIYLLPSubiquitination[1, 2, 4]
375NVSGLTTKNLDYVATubiquitination[1, 2, 3, 4]
390SIHEAVTKIQ*****ubiquitination[1, 2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224] 
Functional Description
  
Sequence Annotation
  
Keyword
 Aminotransferase; Complete proteome; Pyridoxal phosphate; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 392 AA 
Protein Sequence
MQVWSPWKGA MCPRPHKPAY RTDDCHPWVL PVVKKVEQKI ANDNSLNHEY LPILGLAEFR 60
SCASRLALGD DSPALKEKRV GGVQSLGGTG ALRIGADFLA RWYNGTNNKN TPVYVSSPTW 120
ENHNAVFSAA GFKDIRSYRY WDAEKRGLDL QGFLNDLENA PEFSIVVLHA CAHNPTGIDP 180
TPEQWKQIAS VMKHRFLFPF FDSAYQGFAS GNLERDAWAI RYFVSEGFEF FCAQSFSKNF 240
GLYNERVGNL TVVGKEPESI LQVLSQMEKI VRITWSNPPA QGARIVASTL SNPELFEEWT 300
GNVKTMADRI LTMRSELRAR LEALKTPGTW NHITDQIGMF SFTGLNPKQV EYLVNEKHIY 360
LLPSGRINVS GLTTKNLDYV ATSIHEAVTK IQ 392 
Gene Ontology
 GO:0004069; F:L-aspartate:2-oxoglutarate aminotransferase activity; IEA:EC.
 GO:0080130; F:L-phenylalanine:2-oxoglutarate aminotransferase activity; IEA:EC.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0009058; P:biosynthetic process; IEA:InterPro.
 GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro. 
Interpro
 IPR004839; Aminotransferase_I/II.
 IPR000796; Asp_trans.
 IPR004838; NHTrfase_class1_PyrdxlP-BS.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1. 
Pfam
 PF00155; Aminotran_1_2 
SMART
  
PROSITE
 PS00105; AA_TRANSFER_CLASS_1 
PRINTS
 PR00799; TRANSAMINASE.