CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038161
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial 
Protein Synonyms/Alias
  
Gene Name
 Gcat 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
42IRGAGTWKSERVITSacetylation[1, 2]
42IRGAGTWKSERVITSsuccinylation[2]
115IHKNLEAKIAHFHQRubiquitination[3]
184DMADLEAKLKEAQKHacetylation[1, 2, 4, 5, 6, 7, 8]
184DMADLEAKLKEAQKHsuccinylation[2]
184DMADLEAKLKEAQKHubiquitination[3]
186ADLEAKLKEAQKHRLacetylation[8]
323IIQSMAAKTRRFRSKacetylation[2]
323IIQSMAAKTRRFRSKsuccinylation[2]
365QMADDMLKKGKWLTSacetylation[1, 2, 8]
365QMADDMLKKGKWLTSsuccinylation[2]
Reference
 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [8] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Pyridoxal phosphate; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 382 AA 
Protein Sequence
MWASFMWHGA LSPGRRAHSA LAQLRCILDS ELEGIRGAGT WKSERVITSR QGPSIRVDGI 60
SGGILNFCAN NYLGLSSHPA VIQAGLQTLE EFGAGLSSTR FICGTQSIHK NLEAKIAHFH 120
QREDAILYPS CFDANAGLFE ALLTPEDAVL SDELNHASII DGIRLCKAHK YRYRHLDMAD 180
LEAKLKEAQK HRLRLVATDG AFSMDGDIAP LQDICRLAAQ YGALVFVDEC HATGFLGPTG 240
RGTDELLGVM DQVTIINSTL GKALGGASGG YTTGPEPLVS LLRQRSRPYL FSNSLPPAVV 300
GCASKALDLL MESNAIIQSM AAKTRRFRSK MEAAGFTVSG ADHPICPVML GDARLSSQMA 360
DDMLKKGKWL TSRTFRWTAQ GA 382 
Gene Ontology
 GO:0008890; F:glycine C-acetyltransferase activity; IEA:InterPro.
 GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
 GO:0009058; P:biosynthetic process; IEA:InterPro. 
Interpro
 IPR011282; 2am3keto_CoA_ligase.
 IPR001917; Aminotrans_II_pyridoxalP_BS.
 IPR004839; Aminotransferase_I/II.
 IPR015424; PyrdxlP-dep_Trfase.
 IPR015421; PyrdxlP-dep_Trfase_major_sub1.
 IPR015422; PyrdxlP-dep_Trfase_major_sub2. 
Pfam
 PF00155; Aminotran_1_2 
SMART
  
PROSITE
 PS00599; AA_TRANSFER_CLASS_2 
PRINTS