CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015443
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ran-binding protein 10 
Protein Synonyms/Alias
 RanBP10 
Gene Name
 RANBP10 
Gene Synonyms/Alias
 KIAA1464 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
60LPRSWSPKDKYNYIGubiquitination[1]
62RSWSPKDKYNYIGLSubiquitination[1]
236YMREWRAKVQGTVHCubiquitination[1]
293QEEQASIKNRQKIQKubiquitination[1, 2, 3]
300KNRQKIQKLVLEGRVubiquitination[1]
333PNLLFMLKCRQFVEMubiquitination[1]
357SLSSRSPKSQDSYPGubiquitination[1, 2]
480RIVNGAYKHEDLQTDubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Acts as a guanine nucleotide exchange factor (GEF) for RAN GTPase (By similarity). May play an essential role in hemostasis and in maintaining microtubule dynamics with respect to both platelet shape and function (By similarity). May act as an adapter protein to couple membrane receptors to intracellular signaling pathways. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen- induced transactivation. In contrast to RANBP9, does not interact with Sos and does not activate the Ras pathway. 
Sequence Annotation
 DOMAIN 35 222 B30.2/SPRY.
 DOMAIN 253 285 LisH.
 DOMAIN 291 348 CTLH.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 369 369 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 620 AA 
Protein Sequence
MAAATADPGA GNPQPGDSSG GGAGGGLPSP GEQELSRRLQ RLYPAVNQQE TPLPRSWSPK 60
DKYNYIGLSQ GNLRVHYKGH GKNHKDAASV RATHPIPAAC GIYYFEVKIV SKGRDGYMGI 120
GLSAQGVNMN RLPGWDKHSY GYHGDDGHSF CSSGTGQPYG PTFTTGDVIG CCVNLINGTC 180
FYTKNGHSLG IAFTDLPANL YPTVGLQTPG EIVDANFGQQ PFLFDIEDYM REWRAKVQGT 240
VHCFPISARL GEWQAVLQNM VSSYLVHHGY CATATAFARM TETPIQEEQA SIKNRQKIQK 300
LVLEGRVGEA IETTQRFYPG LLEHNPNLLF MLKCRQFVEM VNGTDSEVRS LSSRSPKSQD 360
SYPGSPSLSP RHGPSSSHMH NTGADSPSCS NGVASTKSKQ NHSKYPAPSS SSSSSSSSSS 420
SSPSSVNYSE SNSTDSTKSQ HHSSTSNQET SDSEMEMEAE HYPNGVLGSM STRIVNGAYK 480
HEDLQTDESS MDDRHPRRQL CGGNQAATER IILFGRELQA LSEQLGREYG KNLAHTEMLQ 540
DAFSLLAYSD PWSCPVGQQL DPIQREPVCA ALNSAILESQ NLPKQPPLML ALGQASECLR 600
LMARAGLGSC SFARVDDYLH 620 
Gene Ontology
 GO:0005881; C:cytoplasmic microtubule; IEA:Compara.
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005087; F:Ran guanyl-nucleotide exchange factor activity; IEA:Compara.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR013144; CRA_dom.
 IPR024964; CTLH/CRA.
 IPR006595; CTLH_C.
 IPR006594; LisH_dimerisation.
 IPR013720; LisH_dimerisation_subgr.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt. 
Pfam
 PF10607; CLTH
 PF08513; LisH
 PF00622; SPRY 
SMART
 SM00757; CRA
 SM00668; CTLH
 SM00667; LisH
 SM00449; SPRY 
PROSITE
 PS50188; B302_SPRY
 PS50897; CTLH
 PS50896; LISH 
PRINTS